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D(+)-Raffinose pentahydrate, CAS 17629-30-0, The molecular formula C18H42O21 is a trisaccharide composed of galactose, glucose, and fructose, naturally found in various vegetables and grains. It is a functional oligosaccharide with certain biological activities such as anti-cancer and enhancing immune function. It has a wide range of applications in the production of medicinal chemicals and food additives. It can also be used for identifying intestinal bacteria in bacteriology, and has certain uses in medicine and microbial culture media. As a probiotic for the proliferation of bifidobacteria, it can also be used as a main component of protective delivery fluid for human and animal live organ transplantation. It can also be used as an enhancer and microbial culture medium to prolong the survival of live bacteria at room temperature. Since its launch in the 1990s, the product has been widely used in the food, pharmaceutical, health products, cosmetics, and feed industries.
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Chemical Formula |
C18H42O21 |
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Exact Mass |
594.22 |
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Molecular Weight |
594.51 |
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m/z |
594.22 (100.0%), 595.23 (19.5%), 596.23 (4.3%), 596.23 (1.8%) |
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Elemental Analysis |
C, 36.37; H, 7.12; O, 56.51 |
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D(+)-Raffinose pentahydrate, also known as raffinose or honey trisaccharide, is a functional oligosaccharide with wide application value in the food industry due to its unique physicochemical properties and physiological functions.
It has a certain sweetness and can be used as a sweetener, especially suitable for people who need to control sugar intake, such as diabetes patients. Compared with traditional sweeteners, it not only has a sweet taste, but also has certain health benefits. It can regulate the intestinal microbiota, promote the reproduction of beneficial bacteria, inhibit the growth of harmful bacteria, thereby improving the intestinal environment and enhancing intestinal health.
Application example:
Sugar free food:
It can be used as a sweetener for sugar free food, such as sugar free biscuits, sugar free cakes, etc., to provide delicious sweet enjoyment for diabetes patients.
Functional beverages:
Can serve as sweeteners for functional beverages, such as sports drinks, energy drinks, etc., providing sweetness and energy support for athletes and people in need of energy supplementation.
2. As a food thickener and stabilizer
It has good viscosity and stability in solution and can be used as a food thickener and stabilizer. During the food processing, this substance can increase the viscosity of food, improve its taste and texture, enhance its stability, and extend its shelf life.
Application example:
Yogurt: It can serve as a stabilizer for yogurt, increasing its viscosity and texture, making it more delicate and smooth.
Ice cream: It can serve as a stabilizer for ice cream, increasing its thickness and stability, preventing ice crystals from forming during the freezing process, and maintaining the taste and texture of the ice cream.
Jam: It can be used as a thickener to increase the viscosity and stability of jam, making it richer and more flavorful.
3. As a functional food ingredient
It has multiple physiological functions such as regulating gut microbiota, enhancing immunity, and fighting cancer, and can be used as a raw material for functional foods. By adding this substance, food with specific health functions can be developed to meet consumers' demand for healthy food.
Application example:
Intestinal health products: can be used as raw materials of intestinal health products, such as probiotic powder, intestinal regulator, etc., to improve the intestinal environment and improve the intestinal health level by regulating the intestinal flora.
Immune regulating foods: can be used as raw materials for immune regulating foods, such as oral liquids, capsules, etc., to enhance the body's immunity and improve its ability to resist diseases.
Anti cancer foods: have anti-cancer effects on reproductive cells and can be used as additives to anti-cancer foods, such as anti-cancer oral liquids, anti-cancer capsules, etc., providing adjuvant therapy for cancer patients.
It has a good taste and flavor, and can be used as a food additive to improve the taste and flavor of food. By adding this substance, food can be made sweeter and more delicious, improving its taste and quality.
Application example:
Seasoning: It can be used as an additive to seasonings, such as soy sauce, vinegar, etc., to increase the sweetness and taste of seasonings, making them more delicious.
Baked goods: can be used as additives in baked goods, such as bread, cakes, etc., to increase the sweetness and taste of baked goods, making them softer and sweeter.
Candy: It can be used as a raw material for candy, such as lollipops, chewing gum, etc., to increase the sweetness and taste of candy, making it more delicious and tasty.
D(+)-Raffinose pentahydrate can be used as a feed additive to regulate the intestinal microbiota of animals, improve their growth performance and immunity. In animal husbandry, it can be used as an additive in animal feed, such as pig feed, chicken feed, etc., by improving the intestinal environment of animals, enhancing their digestion and absorption capacity, and promoting their growth and development.
Application example:
Pig feed: can be used as an additive in pig feed, by regulating the intestinal microbiota of pigs, improving their digestion and absorption capacity, promoting their growth and development, and improving the quality and yield of pork.
Chicken feed: can be used as an additive in chicken feed, by regulating the intestinal microbiota of chickens, improving their immunity, reducing the incidence of diseases, and increasing the yield and quality of eggs.
It has unique application value in baking and food processing. Due to its excellent moisturizing properties and thermal stability, it can keep baked goods moist and soft during the baking process, while preventing them from becoming too dry or hard during the baking process.
Application example:
Bread: It can be used as an improver to increase the softness and texture of bread, making it more delicious.
Cake: It can be used as a moisturizer for cakes, keeping them moist and soft during baking, while improving the taste and quality of the cake.
Biscuits: Can serve as sweeteners and moisturizers for cookies, making them more crispy and delicious while maintaining their moisture content.
character
It is a pure natural functional oligosaccharide extracted from plants (sugar beet molasses or cotton seeds) by physical extraction. Cotton seed sugar is a white or light yellow crystalline powder, and the melting point of anhydrous cotton seed sugar is 118-119 ℃. Generally, the crystal contains 5 molecules of crystal water, which will be lost when slowly heated to 100 ℃. The specific optical rotation [α] D of the aqueous solution is+105 °. The melting point of raffinose with crystalline water is 80 ℃. Unlike other oligosaccharides, cottonseed sugar crystal powder contains 5 molecules of crystal water, so it will not absorb moisture or clump even in an environment with a relative humidity of 90%. Compared with other oligosaccharide powders, cottonseed sugar crystal powder has stronger moisture absorption, which is a significant characteristic of cottonseed sugar crystal powder and is extremely beneficial for the addition of sheet-like and powdery foods and medicines. In an amorphous state, it also has strong moisture absorption and moisturizing abilities.
biological activity
It is a functional oligosaccharide with strong proliferation of bifidobacteria, important pharmacological effects, anti hematological effects, and anti-cancer effects on germ cells. In the food industry, it can be added to various beverages, dairy products, nutritional oral liquids, and various health foods. After being consumed by the human body and entering the human colon, it is fermented and utilized by gas producing bacteria to produce intestinal gas, which is also why eating beans (rich in this substance) can cause intestinal gas. It has good food processing properties and is an excellent food base material. It can be used as a substitute for sweeteners for diabetics. D(+)-Raffinose pentahydrate can be made from cottonseed cake flour or beet molasses, and can be taken to regulate intestinal flora.
The analytical methods of D(+)-Raffinose Pentahydrate mainly include the following several types, which have wide applications in purity detection, structure confirmation and impurity analysis, etc.
Purity Detection Methods
High Performance liquid Chromatography (HPLC)
Application: HPLC is a commonly used method for detecting the purity of D(+) -raffinose pentahydrate. By choosing the appropriate chromatographic column and mobile phase, the separation of D(+) -rainose pentahydrate from other impurities can be achieved, thereby accurately determining its purity.
Features: HPLC is characterized by high separation efficiency, fast analysis speed and high sensitivity, and can accurately determine the content and purity of D(+) -rainose pentahydrate.
Mass Spectrometry
Application: Mass spectrometry can be used to confirm the molecular weight information of D(+) -rainose pentahydrate and conduct impurity analysis. Through mass spectrometry, the molecular ion peaks and impurity peaks of D(+) -rainose pentahydrate can be directly observed, thereby determining its purity.
Characteristics: Mass spectrometry is characterized by high sensitivity and high resolution, and can provide molecular weight information of compounds. It is of great significance for confirming the structure of compounds and analyzing impurities.
Methods for Structural Confirmation
Nuclear magnetic resonance method (NMR)
Application: NMR can provide information on the relative positions and chemical environment of atoms in D(+) -raffinose pentahydrate, which is very useful for confirming its three-dimensional structure. Through NMR spectra, the chemical shifts of each hydrogen and carbon atom in D(+) -raffinose pentahydrate can be analyzed, thereby inferring its molecular structure.
Characteristics: NMR is non-destructive, has high resolution and can provide rich structural information, making it an important means to confirm the structure of compounds.
Impurity Analysis Methods
Thin-layer chromatography (TLC)
Application: TLC can be used for the preliminary separation and detection of impurities in D(+) -raffinose pentahydrate. By choosing the appropriate developing agent and chromogenic agent, the separation and visual detection of D(+) -raffinose pentahydrate from impurities can be achieved.
Features: TLC is characterized by its simplicity, rapidity and high sensitivity, and is suitable for the separation and detection of various compounds.
Capillary electrophoresis method
Application: Capillary electrophoresis is an efficient and rapid separation technique that can be used for the separation and detection of impurities in D(+) -raffinose pentahydrate. By choosing appropriate electrophoresis conditions and detectors, efficient separation and accurate detection of D(+) -rainose pentahydrate and impurities can be achieved.
Features: Capillary electrophoresis has the characteristics of high separation efficiency, fast analysis speed and small sample size, and is suitable for the separation and detection of impurities in complex samples.
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