Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of methylene blue dye powder cas 61-73-4 in China. Welcome to wholesale bulk high quality methylene blue dye powder cas 61-73-4 for sale here from our factory. Good service and reasonable price are available.
Methylene blue dye powder, also known as methylene blue, is a phenothiazine salt, CAS C16H18ClN3S, The chemical formula is C16H18N3ClS. It is a glossy dark green bronze crystal or powder. It is relatively stable in the air. It forms complex salts with most inorganic salts, and its aqueous solution is alkaline and toxic. Soluble in water and ethanol, the aqueous solution is alkaline and insoluble in ether. Adding dilute sulfuric acid to methylene blue solution will cause the solution to fade. If quickly added to ammonia or exposed to air, it can be restored and widely used as a chemical indicator (methylene blue indicator), dye, biological dye, and drug. Belonging to the dye class compounds, industrial grade methylene blue is often used for dyeing cotton, silk, paper, etc. It can also be applied in coloring bamboo and wood, as well as manufacturing inks, color lakes, etc. In addition, it is often used as a bacterial stain, indicator, etc.
|
Chemical Formula |
C16H18ClN3S |
|
Exact Mass |
319 |
|
Molecular Weight |
320 |
|
m/z |
319 (100.0%), 321 (32.0%), 320 (17.3%), 322 (5.5%), 321 (4.5%), 323 (1.4%), 321 (1.4%), 320 (1.1%) |
|
Elemental Analysis |
C, 60.08; H, 5.67; Cl, 11.08; N, 13.14; S, 10.02 |
|
|
|
Melting point 190 °C ( dec. ) ( lit. ) , Density 1.0 g / mL at 20 ° C , Refractive index n20 / D1.347 , flash point 45 °C , Storage condition room temp , solubility in water, ethanol, ethylene glycol, methyl cellosolve , Acidity coefficient ( pKa ) 2.6,11.2 ( at 25 °C ) , Morphological Liquid , Color index 52015 , Color Green , Proportion 0.98 , Odor ( Odor ) Odorless , Water solubility 40 g / L ( 20 oC ) , Maximum wavelength ( λmax ) 661 nm , BRN 3641570 , Application of microbial detection in biological field ; diabetic retinopathy, macular degeneration, malignant uveal melanomas, erysipelas, hidradenitis obliteraa, inflammation, skin diseases.
|
|
|
Methylene blue dye powder, also known as 3,7-bis (dimethylamino) phenothiazine-5-ium chloride trihydrate, is a dark green crystalline powder with a bronze luster that is easily soluble in water and ethanol. Its aqueous solution appears blue. As a synthetic dye with a long history, since its first synthesis in the late 19th century, it has gradually expanded from the field of textile printing and dyeing to multiple disciplines such as aquaculture, pharmaceutical diagnosis, biochemistry, etc., becoming a classic compound for cross disciplinary applications.
1. Prevention and treatment of fungal diseases
It is the preferred drug for preventing and treating Saprolegniasis in aquaculture. Water mold is caused by fungi of the water mold genus, commonly found at low water temperatures (10-18 ℃). After the fish body is injured, fungal spores attach to the wound, forming white cotton like hyphae. In severe cases, it can cause the fish body to rot and die. By disrupting the permeability of fungal cell membranes and inhibiting their metabolic activity, the disease can be effectively controlled.
Application case:
In ornamental fish farming, it is commonly used for quarantine treatment of new fish before entering the tank. Soaking fish bodies in a 0.1-0.2 ppm (mg/L) methylene blue solution for 15-30 minutes can kill surface fungal spores and reduce the risk of infection.
For diseased fish populations, the whole pond spraying method can be used, using 2-3 grams of methylene blue per cubic meter of water, once every other day for 2-3 consecutive times, with a cure rate of over 80%.
2. Parasitic disease treatment
It has significant therapeutic effects on parasitic infections such as Ichthyophthoriasis and Trichodiniasis. Ichthyophthorius multifiliis is a large protozoan parasite whose larvae burrow into the skin of fish to form white vesicles, causing itching and friction on the vessel walls, and in severe cases, secondary infections. By interfering with the parasite's respiratory chain and inhibiting its energy metabolism, it kills the parasite.
Application case:
When treating small melon worm disease, the concentration of methylene blue can be increased to 0.3-0.5 ppm and sprayed throughout the pool once a day for 3-5 consecutive days. Simultaneously increasing the temperature to above 30 ℃ (as small melon worms cannot survive at high temperatures) can significantly shorten the treatment course. For wheel worm disease, methylene blue is often combined with copper sulfate to enhance the deworming effect.
3. Water quality improvement and detoxification
It has redox properties and can regulate the oxidation-reduction potential (ORP) of water bodies, promoting the conversion of harmful substances such as ammonia nitrogen and nitrite.
In addition, it can also combine with heavy metal ions (such as copper and lead) in water to form insoluble precipitates, reducing toxicity.
Application case:
In intensive farming mode, it is commonly used to alleviate nitrite poisoning. When the concentration of nitrite in water exceeds 0.1 ppm, sprinkling methylene blue at a dose of 1 gram per cubic meter of water can quickly reduce the toxicity of nitrite and buy time for water replacement. At the same time, the blue staining effect of methylene blue can increase the transparency of water bodies, making it easier to observe the activity status of fish.
4. Disinfection and hatching promotion of fish eggs
Can be used for surface disinfection of fish eggs to prevent fungal and bacterial infections. Its mild medicinal properties do not damage fish egg embryos, but instead promote embryo development by improving water quality conditions (such as increasing dissolved oxygen levels).
Application case:
In the artificial reproduction of freshwater fish such as carp and crucian carp, soaking newly produced fish eggs in a 0.05 ppm methylene blue solution for 5 minutes can significantly improve hatching rate. For sticky eggs (such as golden fish eggs), methylene blue solution can be sprayed onto the attached substrate to form a protective film and prevent fungal growth.
Medical field: Cross border applications from dyes to life-saving drugs
1. A specific antidote for methemoglobinemia
Methylene blue dye powder is a blood disorder caused by the oxidation of iron ions in hemoglobin to trivalent iron (Fe ³ ⁺), resulting in a loss of oxygen transport capacity. The patient presents with cyanosis of the skin and mucous membranes, difficulty breathing, and in severe cases, coma or even death. As a reducing agent, it can reduce methemoglobin to normal hemoglobin (Fe ² ⁺) and restore oxygen carrying function.
Mechanism of action:
Reduced to "Leucomethylene Blue" in the body, which acts as an electron donor to reduce Fe ³ ⁺ in methemoglobin to Fe ² ⁺. At the same time, it can activate the NADPH dependent reductase system, accelerating the metabolic clearance of methemoglobin.
Clinical case:
In 2023, a nitrite leakage accident occurred in a chemical plant, and multiple workers developed methemoglobinemia after inhaling high concentrations of nitrite gas. The emergency department doctor immediately administered 1% methylene blue injection (1-2 mg/kg body weight) intravenously, and after 10 minutes, the patient's cyanosis symptoms significantly improved, and blood oxygen saturation returned to the normal range.
2. Emergency medication for cyanide poisoning
Cyanide (such as HCN, KCN) poisoning can cause interruption of the intracellular respiratory chain, leading to tissue hypoxia. Methylene blue promotes the combination of cyanide and sodium thiosulfate to form non-toxic thiocyanate by providing electrons, thereby relieving toxicity.
Mechanism of action:
As an electronic carrier, cyanide is dissociated from cytochrome oxidase to restore enzyme activity. At the same time, it can enhance the detoxification effect of sodium thiosulfate, forming a "methylene blue sodium thiosulfate" combination therapy.
Clinical case:
In 2024, a potassium cyanide ingestion incident occurred in a university laboratory, causing the patient to quickly become unconscious and experience respiratory depression. The emergency team immediately administered intravenous injection of methylene blue (5 mg/kg body weight) combined with sodium thiosulfate (150-200 mg/kg body weight), and the patient's consciousness gradually recovered with stable vital signs.
3. Antibacterial and antifungal therapy
It has broad-spectrum antibacterial activity and has inhibitory effects on Gram positive bacteria (such as Staphylococcus aureus), Gram negative bacteria (such as Escherichia coli), and some fungi (such as Candida albicans). Its antibacterial mechanism includes disrupting cell membrane structure, inhibiting DNA replication, and interfering with energy metabolism.
Clinical application:
Urinary tract infection: Oral methylene blue (100-200 mg/day) or bladder instillation (0.1% solution) can be used to treat cystitis and urethritis, especially for patients who are resistant to antibiotics.
Oral infection: Methylene blue mouthwash (0.05% concentration) can alleviate symptoms such as oral ulcers and gingivitis, and reduce bacterial colonization.
Skin infection: Methylene blue ointment (1-2% concentration) can be used topically for burn wounds, bedsores, etc., to prevent infection and promote healing.
4. Indicators for renal function assessment
Methylene blue is rapidly excreted through the kidneys after absorption in the body, and its excretion rate is closely related to glomerular filtration rate (GFR). By measuring the concentration changes of methylene blue in urine, renal function can be indirectly evaluated.
Testing method:
After intravenous injection of methylene blue (1 mg/kg body weight), urine samples were collected at regular intervals to measure their absorbance or fluorescence intensity and calculate the clearance rate. The normal clearance rate of methylene blue in adults is 100-150 ml/min, and it is significantly reduced in patients with renal dysfunction.
5. Potential drugs for anti-cancer research
In recent years, it has demonstrated unique value in the field of tumor treatment. Its photodynamic therapy (PDT) can be activated by specific wavelength lasers to generate reactive oxygen species such as singlet oxygen, selectively killing tumor cells. In addition, it can also inhibit tumor angiogenesis and block nutrient supply.
Research progress:
In 2025, a team published research in Nature Medicine, which confirmed that methylene blue nanoparticles (MB NPs) can be delivered to tumor tissues. Through the synergistic effect of photothermal effect and chemical kinetic therapy, they can significantly inhibit the growth of breast cancer in mice, and have very low toxicity to normal tissues.
Methylene blue dye powder, as a cross century 'blue miracle', has witnessed the deep integration of chemistry and life sciences in its application history. From saving aquatic animals to saving human lives, from dye workshops to operating rooms, this classic compound continues to shine with scientific charm, contributing unique value to human health and sustainable development.
Hot Tags: methylene blue dye powder cas 61-73-4, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, mercury indicator powder, Dithizone Indicator, Neutral red powder, Chloramine T powder, Chemical Reagent, Sodium Diethyldithiocarbamate