Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of decapeptide-12 cream in China. Welcome to wholesale bulk high quality decapeptide-12 cream for sale here from our factory. Good service and reasonable price are available.
Decapeptide-12 cream, as a "dark horse" in the field of cosmetic raw materials in recent years, has demonstrated outstanding efficacy in whitening, spot removal, anti-aging, and skin repair due to its unique molecular structure and multi-target mechanism of action. It achieves comprehensive intervention from the molecular level to the tissue level by precisely regulating the melanin generation pathway, activating the skin repair signaling network, and optimizing the active ingredient delivery system, becoming one of the core components in high-end skincare formulas.
DecaPeptide-12 COA
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| Certificate of Analysis | ||
| Compound name | DecaPeptide-12 | |
| Grade | Pharmaceutical grade | |
| CAS No. | 137665-91-9 | |
| Quantity | 337.3kg | |
| Packaging standard | 25kg/drum | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | 202501090049 | |
| MFG | Jan 9th 2025 | |
| EXP | Jan 8th 2028 | |
| Structure |
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| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.49% |
| Loss on drying | ≤1.0% | 0.32% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.90% |
| Single impurity | <0.8% | 0.47% |
| Total microbial count | ≤750cfu/g | 80 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 500ppm |
| Storage | Store in a sealed, dark, and dry place below 2-8°C | |
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Molecular properties and functional basis
It is a short peptide chain composed of 10 amino acids with a molecular weight of 1395.52 Da, which combines the permeation advantages of small molecule peptides with the targeting properties of biologically active peptides. Its molecular structure contains tyrosine (Tyr) and tryptophan (Trp) residues, which can competitively bind to the active site of tyrosinase by simulating the benzene ring structure of tyrosine, directly blocking the key step of melanin synthesis. In addition, the charge distribution and spatial conformation enable it to efficiently penetrate the stratum corneum of the skin, targeting melanocytes and fibroblasts, laying the foundation for its multifunctional function.
Core mechanism of action: Dual pathway reshaping of skin health
1. Targeted inhibition of melanin production for efficient whitening
Intervention of melanin synthesis through the dual pathway of "competitive inhibition+signal regulation":
Direct inhibition of tyrosinase activity: Experiments have shown that its inhibition constant (Kd) is 61.1 μ M, and the half inhibitory concentration (IC50) is 40 μ M. At a concentration of 100 μ M, Decapeptide-12 cream can reduce melanin content in melanocytes by 43% without affecting cell proliferation.
Downregulation of MITF expression: By inhibiting the transcription factors associated with microphthalmia (MITF), the synthesis of key enzymes such as tyrosinase and TRP-1 is indirectly reduced, forming a long-lasting whitening mechanism.
Blocking the α - MSH signal: Inhibiting the binding of melanocyte stimulating hormone (α - MSH) to melanocortin 1 receptor (MC1R), cutting off the melanin production signal upstream. Clinical verification shows that its whitening effect is equivalent to 4% hydroquinone, but its irritability is significantly reduced.
2. Activate the Sirtuins family to reverse skin aging
By upregulating the expression of "longevity proteins" such as SIRT1, SIRT3, and SIRT6, aging can be delayed from three aspects: cellular metabolism, DNA repair, and collagen synthesis
Promoting collagen synthesis: In artificial skin models, it increases the content of type I and III collagen by 29% and increases the density of elastic fibers by 18%.
Inhibition of matrix metalloproteinases (MMPs): reduces the expression of collagenase (MMP-1) and prevents collagen degradation.
Enhance mitochondrial function: improve cellular energy metabolism and reduce oxidative stress damage through SIRT3 activation.
Clinical data shows that after 12 weeks of use, eye cream containing 0.01% Decapeptide-12 reduces the depth of wrinkles around the eyes by 14% and improves skin firmness by 19%.
Technological breakthrough: Delivery system and formula optimization
To overcome the limitations of the skin barrier, liposome encapsulation, nanostructured lipid carriers (NLC), and microneedle patch technology are commonly used
Liposome encapsulation: Transdermal absorption rate increased by 3.2 times, dermal retention increased by 2.8 times, and sustained release of active substances lasted for 12 hours.
NLC technology: in the accelerated test at 40 ℃, the active substance retention rate reached 92%, the penetration depth increased by 40%, and the stability was significantly better than that of ordinary cream.
Micro needle patch: the active substance is delivered directly to the dermis, and the therapeutic effect on chloasma is 39% higher than that of cream, and the depth of action can reach the dermal papilla layer.
In addition, it is often combined with nonapeptide-1 (blocking alpha MSH signaling), chalcopeptide (promoting collagen repair), or antioxidants (such as vitamin C) to form a synergistic system of "whitening+anti-aging+repair". Clinical tests have shown that its comprehensive effect is improved by more than 40%.
Decapeptide-12 cream, as a star ingredient in the field of cosmetic raw materials, has shown significant advantages in whitening, freckle removal, anti-aging, and skin barrier repair due to its unique dual mechanism of action - targeted inhibition of melanin production and activation of skin repair pathways. Its mechanism of action involves multiple levels such as enzyme activity regulation, gene expression intervention, cell signaling, and skin structure remodeling, forming a multidimensional intervention network from molecules to tissues.
1. Competitive binding of tyrosinase active site
Tyrosinase is a key rate limiting enzyme in melanin synthesis, and its active center contains two copper ion (Cu ² ⁺) binding sites, responsible for catalyzing the conversion of tyrosine to dopaquinone. By simulating the benzene ring structure of tyrosine through the residues of tyrosine (Tyr) and tryptophan (Trp) in its molecular structure, it competes with the C-terminal residue of tyrosinase to directly block the enzyme's contact with the substrate. The experimental data showed that the inhibition constant (Kd) of mushroom tyrosinase was 61.1 μ M, and the half maximal inhibitory concentration (IC50) was 40 μ M, indicating its high inhibitory ability. In human melanocyte experiments, treatment with Decapeptide-12 at a concentration of 100 μ M for 7 days resulted in a 43% decrease in melanin content without affecting cell proliferation, confirming its safety and effectiveness.
2. Multi target inhibition of melanin production pathway
In addition to directly inhibiting tyrosinase activity, it can also achieve long-lasting whitening by regulating signaling pathways related to melanin production. Research has shown that this peptide can downregulate the expression of microphthalmia related transcription factor (MITF), which is a core regulatory factor for melanocyte differentiation and tyrosinase gene expression. By inhibiting MITF, the expression of key melanin synthesis enzymes such as tyrosinase and TRP-1 (tyrosinase related protein 1) is indirectly reduced, forming a "dual inhibition" mechanism. In addition, it can inhibit the binding of α - MSH (melanocyte stimulating hormone) and MC1R (melanocortin 1 receptor), block the upstream signal transduction of melanin production, and further consolidate its whitening effect.
3. Clinical validation: Improvement of melasma and photoaging
In a double-blind controlled trial for 30 Asian women, after 8 weeks of use of the cream containing 0.01% Decrepeptide-12, the area of chloasma was reduced by 31% on average, and the intensity of pigment was reduced by 27%. The effect was equivalent to that of 4% hydroquinone cream, but the irritation was significantly reduced (15% of the hydroquinone group had erythema and scaling). Another open trial targeting patients with photoaging showed that after using the compound formula (containing antioxidants and fruit acids) for 3 months, 38.5% of patients' photoaging grading decreased from grade III to grade I, and the arrangement of elastic fibers in the dermis layer significantly improved, proving that it has both whitening and anti-aging effects.
Sirtuin family activation: the core driver of skin repair and anti-aging
1. Biological functions of Sirtuins
Sirtuins (SIRT1-7) are a class of NAD ⁺ - dependent histone deacetylases, known as "longevity proteins," that play critical roles in cellular metabolism, DNA repair, inflammation regulation, and aging processes. Among them, SIRT1 can promote collagen synthesis and inhibit the activity of matrix metalloproteinases (MMPs), thereby improving skin elasticity; SIRT3 reduces oxidative stress and delays cellular aging by regulating mitochondrial function; SIRT6 is involved in DNA damage repair and maintains genomic stability.
2. The regulatory effect of Decapeptide-12 on Sirtuins
Experiments have shown that Decapeptide-12 (100 μ M, 72 hour treatment) can significantly upregulate the transcription levels of SIRT1, SIRT3, SIRT6, and SIRT7 in human neonatal keratinocytes without causing cytotoxicity. Further research has found that the peptide indirectly enhances Sirtuins activity by activating the AMPK (adenosine monophosphate activated protein kinase) signaling pathway, increasing intracellular NAD ⁺ levels. In the artificial skin model, Decapeptide-12 promotes the secretion of type I and III collagen by fibroblasts, while inhibiting the expression of MMP-1 (collagenase), resulting in a 29% increase in collagen content and an 18% increase in elastic fiber density, significantly improving skin structure.
3. Clinical evidence of anti-aging
A randomized controlled trial involving 60 subjects showed that after using an eye cream containing 0.01% Decapeptide-12 cream for 12 weeks, the depth of wrinkles around the eyes decreased by 14%, the skin tightness increased by 19%, and there were no irritating reactions. Organizational analysis shows that collagen fibers in the dermis are arranged more tightly and the elastic fiber network structure is more complete, proving that it reverses skin aging at the cellular level.
1. Liposome encapsulation technology
The molecular weight is 1395.52 Da, and although it has a certain transdermal ability, its bioavailability can be further enhanced by liposome encapsulation. Liposomes are composed of phospholipid bilayers, similar in structure to skin cell membranes, and can directly deliver active substances to keratinocytes or melanocytes through fusion. The experiment shows that the transdermal absorption rate of liposome coated cream is 3.2 times higher than that of ordinary cream, and the retention amount in the skin stratum corneum is 2.8 times higher, and the active substance is continuously released for 12 hours.
2. Nanostructured lipid carriers (NLC)
NLC uses a mixed matrix of solid and liquid lipids to further enhance its stability. In the 40 ℃ accelerated test, the active substance retention rate (92%) of the NLC formula group was significantly higher than that of the ordinary cream (78%), and the penetration depth in the skin increased by 40%. In addition, the sustained-release properties of NLC can reduce the waste of active substances in the epidermal layer and lower the risk of irritation.
3. Micro needle SMT technology
For deep pigmentation (such as melasma), microneedle patches can directly deliver it to the dermis layer. Animal experiments showed that the skin melanin content of the microneedle group was 39% lower than that of the cream group, and the depth of action could reach the dermal papilla layer. This technology breaks through the stratum corneum barrier through physical puncture, forming microchannels that allow active substances to bypass the epidermal barrier and reach the target site, significantly improving treatment efficacy.
Synergistic formula: Multi target joint intervention strategy
1. Whitening Gold Combination: Decapeptide-12+Nine Peptide-1
Nonapeptide-1 blocks upstream signaling of melanin production by inhibiting the binding of α - MSH to MC1R. When combined with Decapeptide-12, the two form a dual pathway whitening mechanism of inhibition blockade. Clinical tests have shown that this combination improves whitening effectiveness by 47% and shortens the onset time to 4 weeks.
2. Anti aging composite system: Decapeptide-12+Blue Copper Peptide
GHK Cu can promote collagen synthesis, anti-inflammatory effects, and repair the skin barrier.
When combined with Decapeptide-12, the former is responsible for repairing photodamaged skin, while the latter inhibits melanin production, forming a synergistic effect of "whitening+anti-aging". In a trial of 60 subjects, this combination increased skin brightness by 22%, reduced wrinkle volume by 18%, and was well tolerated.
3. Stability enhancement formula: Decapeptide-12+antioxidant
Antioxidants such as vitamin C and vitamin E can neutralize free radicals, reduce oxidative stress on melanocytes, and protect them from light and heat degradation. The experiment showed that the active matter retention rate of the cream containing 1% vitamin C increased to 95% and the whitening effect increased by 30% in the accelerated test at 40 ℃.
Safety and Tolerance: Validation from Laboratory to Real World
1. Cytotoxicity testing
In the 3T3 fibroblast model, the IC50 value of Decapeptide-12 cream is greater than 1000 μ M, which is much higher than the effective concentration (0.01-0.1 μ M), indicating that it has no cytotoxicity. In addition, the peptide did not exhibit mutagenicity or allergenicity, meeting the safety standards for cosmetic raw materials.
2. Human body patch test
A 48 hour closed patch test was conducted on 200 subjects of different skin types, and only 1 case showed mild erythema (0.5%), which disappeared spontaneously within 24 hours, confirming its suitability for all skin types, including sensitive skin. Long term use (12 weeks) did not cause contact dermatitis or pigmentation rebound.
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