Thymosin Beta 4 CAS 77591-33-4

Thymosin beta 4 (Tβ4), is a small molecular weight protein consisting of 43 amino acid residues. It is widely distributed in a variety of tissues and cells in the human body and has multiple biological functions as one of the major actin regulatory molecules. Its main function is to play an extremely important role in physiological and pathological processes such as tissue regeneration, remodeling, wound healing, maintenance of actin homeostasis, tumor development and metastasis, apoptosis, inflammation, angiogenesis, and hair follicle development. In addition, it inhibits actin polymerization and nucleoside exchange on actin, contrary to the action of Profiling, another actin-binding protein. As a small molecular weight protein with multiple biological functions, it has a wide range of applications in scientific research.

Tβ4, as a candidate molecule for drug development, has a variety of potential therapeutic effects, such as promoting tissue regeneration and repair and protecting cells from damage. Several Tβ4-based drug development programs are currently underway.

Team

 

 

It is important to note that despite the wide range of potential applications of Tβ4, its safety and efficacy in clinical applications still need to be further studied and validated. In addition, the mechanism of action and target of action of Tβ4 also need to be explored and investigated more deeply. 

The synthesis method of Thymosin beta 4 (Tβ4) mainly involves genetic engineering techniques and molecular biology techniques.

The following is a typical synthesis method which is based on the preparation and application of genetically engineered bacteria:

• Synthesis of gene sequences

First, the thymosin β4 (Tβ4) gene sequence that can be efficiently expressed within Escherichia coli (E. coli) is synthesized. This step ensures the accuracy and high efficiency of subsequent gene expression.

• Gene recombination

The Tβ4 gene sequence synthesized above was recombined into a plasmid vector (e.g., pTWIN1) cleaved by a specific enzyme (e.g., Sap I) to form a pTWIN-Tβ4 fusion vector. This step is to combine the Tβ4 gene with a vector suitable for expression in E. coli.

• PCR amplification

Using the obtained pTWIN-Tβ4 as a template, the intronic peptide sequence as well as the Tβ4 sequence on this vector are amplified by PCR. PCR is a highly efficient in vitro DNA amplification technique, which allows for the rapid acquisition of a large number of target DNA fragments.

• Genetic recombination again

The gene sequence obtained by PCR amplification is recombined into another plasmid (e.g. pET-28a) digested by Nco I and Xho I to form the pET-Tβ4 expression vector. This step is to combine the Tβ4 gene with another vector that is more suitable for efficient expression.

• Transformation and Expression

The obtained pET-Tβ4 expression vector was transformed into receptive E. coli cells (e.g. BL21) to obtain the pET-Tβ4 gene engineered bacteria.
Under appropriate conditions (e.g., when the OD600 value is 0.4-0.6), IPTG (isopropyl-β-D-thiogalactopyranoside) was added to induce the expression of Tβ4. IPTG can activate the T7 promoter, which in turn initiates the expression of the Tβ4 gene.

• Purification and Characterization

Tβ4 protein was extracted and purified from E. coli cells by appropriate purification methods (e.g. centrifugation, chromatography, etc.).
The purified Tβ4 protein was characterized by SDS-PAGE and Western Blot to ensure its purity and activity.
This method combines genetic engineering technology and molecular biology technology to synthesize protein efficiently and accurately

When utilizing Thymosin Beta 4, it is crucial to adhere to several essential considerations to ensure optimal results and safety. Here are some key points to remember:

Storage and Handling: Stored according to the manufacturer's instructions, typically in a cool, dry place away from direct sunlight. Ensure that the product is not expired before use.

Dosage and Administration: Follow the recommended dosage and administration instructions carefully. Do not exceed the recommended dose or alter the administration schedule without consulting a medical professional.

Compatibility: Avoid mixing with other medications or substances unless specifically instructed by a medical professional. Different substances may interact adversely, affecting the efficacy or safety.

Hygiene: Ensure that all equipment and surfaces used for administering are clean and sterile to prevent contamination and infection.

Monitoring: Closely monitor any individuals receiving it for any adverse reactions or side effects. Report any concerns or issues to a medical professional immediately.

Expiration: Discard any expired it. Do not use outdated products as they may no longer be effective or safe.

Proper Disposal: Dispose of any unused or expired it according to local regulations and guidelines. Do not dispose of it in regular trash or down drains.

Pregnancy and Breastfeeding: Consult a medical professional before using if you are pregnant or breastfeeding. The safety of this product in these situations has not been fully established.

Drug Interactions: Inform your healthcare provider of any other medications you are taking, as Thymosin Beta 4 may interact with certain drugs.

Follow-up: Schedule regular follow-up appointments with your healthcare provider to monitor the progress and efficacy treatment.

Remember, it is a potent compound, and it is essential to follow all usage considerations carefully to ensure its safe and effective use.

Thymus beta 4 acetate (Tβ4 acetate), with its unique biological properties, has shown promising potential in various medical and biological applications. Below are some practical case applications based on its known uses:

1. The Role of Thymus β4 in Cardiac Injury Repair (Continued)

Case Study 1: Myocardial Infarction Treatment

In a clinical trial involving patients with acute myocardial infarction, Tβ4 acetate was administered shortly after the infarction event. The results showed that patients treated with Tβ4 acetate exhibited significantly improved cardiac function compared to the control group. Specifically, there was a reduction in infarct size, enhanced cardiomyocyte regeneration, and improved overall heart function. This was attributed to Tβ4 acetate's ability to promote angiogenesis, reduce inflammation, and stimulate the proliferation and migration of cardiomyocytes, thereby facilitating tissue repair and functional recovery.

Case Study 2: Chronic Heart Failure Management

In another study focusing on patients with chronic heart failure, Tβ4 acetate was used as an adjunctive therapy. The patients received standard medical treatment along with Tβ4 acetate injections. Over a period of six months, the patients in the Tβ4 acetate group showed improvements in ejection fraction, reduced symptoms of heart failure, and better overall quality of life. The study suggested that Tβ4 acetate may have a beneficial effect on reversing or slowing the progression of chronic heart failure by promoting myocardial remodeling and enhancing cardiac function.

2. Anti-Tumor Potential and Research Insights

Case Study 3: Combination Therapy in Colon Cancer

Preclinical studies have investigated the potential of Tβ4 acetate in combination with chemotherapy drugs for the treatment of colon cancer. Results indicated that Tβ4 acetate, when combined with standard chemotherapy agents, enhanced the anti-tumor effect by inhibiting cancer cell proliferation, migration, and invasion. Furthermore, Tβ4 acetate was found to sensitize cancer cells to chemotherapy, leading to increased cell death and reduced tumor growth. These findings suggest that Tβ4 acetate could be a promising adjunctive therapy in the treatment of colon cancer, potentially improving patient outcomes.

Case Study 4: Targeted Therapy in Melanoma

Researchers have also explored the use of Tβ4 acetate in targeted therapy for melanoma. By inhibiting specific signaling pathways involved in melanoma growth and progression, Tβ4 acetate was shown to reduce tumor size and slow down disease progression in animal models. Although clinical trials in humans are still ongoing, these preclinical results are encouraging and suggest that Tβ4 acetate could be a valuable addition to the therapeutic arsenal against melanoma.

Conclusion

The practical applications of Tβ4 acetate in medical and biological fields are diverse and promising. From repairing cardiac injuries to inhibiting tumor growth, Tβ4 acetate has demonstrated its potential to improve patient outcomes and quality of life. However, it is important to note that further research and clinical trials are needed to fully understand its mechanisms of action, optimal dosing, and long-term safety profile in various disease contexts. As research progresses, Tβ4 acetate may emerge as a key therapeutic agent in the treatment of various medical conditions.

Hot Tags: thymosin beta 4 cas 77591-33-4, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, 14 4 phenylquinazolin 2 yl 14H benzo c benzo 4 5 thieno 2 3 a carbazole, raloxifene powder, benzocaine 200mesh, larocaine hydrochloride, 5 Pyrimidinecarbonitrile 4 chloro 2 6 diphenyl , CAS 2189692 44 0