Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of bis-tris propane cas 64431-96-5 in China. Welcome to wholesale bulk high quality bis-tris propane cas 64431-96-5 for sale here from our factory. Good service and reasonable price are available.
BIS-Tris propane from Bloomtechz appears as a white crystalline powder and has excellent water solubility. High-concentration aqueous solutions are colorless and transparent. This unique dissolving property gives it a standout advantage in the preparation of biological buffers. Water is the most fundamental and common solvent in biological systems, and good water solubility ensures that this buffer raw material diffuses evenly in biological systems, maintaining a stable buffering effect. It's suitable for biochemical experiments, protein purification, cell-related research, and other applications.
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Chemical Formula |
C11H26N2O6 |
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Exact Mass |
282 |
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Molecular Weight |
282 |
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m/z |
282 (100.0%), 283 (11.9%), 284 (1.2%) |
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Elemental Analysis |
C, 46.80; H, 9.28; N, 9.92; O, 34.00 |
On the production control side, Bloomtechz completes the entire process of synthesis, crystallization, recrystallization, drying, grinding, and packaging in cleanrooms dedicated to biochemical reagents. As a high-end biological buffer raw material, the product has strict requirements for impurities, residual solvents, and water solubility. The production area continuously controls temperature and humidity, collects dust, and prevents static electricity to avoid dust, cross-contamination, and to ensure stable purity and solubility. Every batch undergoes a full set of tests after preparation, checking appearance, purity, clarity of aqueous solutions, drying loss, and other key indicators, and a COA quality inspection certificate is issued to ensure clear, transparent solutions without turbidity or insoluble matter, reliably meeting overseas customers' standards for biochemical experiments and buffer preparation. Packaging is completed in a closed workstation, reducing long-term contact between the powder and air, lowering the risk of moisture absorption, and safeguarding product quality from the source.
BIS-Tris Propane (chemical formula: C11H26N2O6, CAS number: 64431-96-5), also known as Bis Tris Propane, is a white to off white crystalline powder with a melting point of 164-165 ℃, a density of 1.3 ± 0.1 g/cm ³, and good water solubility (1 M, 20 ℃). As an important member of the TRIS series of biological buffering agents, its core applications are concentrated in the fields of biochemistry, molecular biology, and laboratory research. The following provides a systematic analysis of its technical principles, application scenarios, and operational points.
1. Buffer range and mechanism
The buffering range of 1,3-Bis ((trihydroxymethyl) methylamino) propane is pH 6.3-9.5, with two acid dissociation constants (pKa ₁=6.8, pKa ₂=9.0) at 25 ℃. Its zwitterionic properties make it excellent in weakly acidic to neutral environments, effectively resisting external acid-base interference through proton exchange reactions and maintaining solution pH stability. For example, in a biochemical diagnostic kit, it can prevent enzyme inactivation or protein denaturation caused by pH fluctuations, ensuring the reproducibility of experimental results.
2. Application of PCR diagnostic reagents
1,3-Bis ((trihydroxymethyl) methylamino) propane is a commonly used buffer component in polymerase chain reaction (PCR), and its advantages include:
Enhance the activity of restriction endonucleases: By optimizing the pH environment, improve enzyme cleavage efficiency and reduce non-specific cleavage. For example, in gene cloning experiments, the use of 1,3-Bis ((trihydroxymethyl) methylamino) propane buffer can improve the cutting accuracy of restriction endonucleases by more than 30%.
Compatibility: No interaction with PCR reaction components such as Mg ² ⁺ and dNTP, to avoid inhibiting amplification reactions. Experiments have shown that in the PCR system containing 1,3-Bis ((trihydroxymethyl) methylamino) propane, the amplification efficiency of the target DNA fragment can reach over 95%.Thermal stability: Maintain structural stability during the high-temperature denaturation step (95 ℃) of the PCR cycle, without decomposing to produce interfering substances.
After long-term high-temperature treatment, its buffering capacity only decreases by 5% -8%, significantly better than traditional buffering agents.
3. DNA/RNA Extraction Kit
The buffering effect of 1,3-Bis ((trihydroxymethyl) methylamino) propane in cell lysate can protect nucleic acids from extreme pH damage and improve extraction purity. For example, in plant genome DNA extraction, the use of 1,3-Bis ((trihydroxymethyl) methylamino) propane buffer can increase DNA yield by 20% and reduce RNA contamination rate to below 1%.
1. Protein research
Study on the Autooxidation of Oxymyoglobin (MbO ₂): 1,3-Bis ((trihydroxymethyl) methylamino) propane as a buffer can investigate the effect of different nucleophilic anions (such as Cl ⁻, NO ∝⁻) on the rate of MbO ₂ auto oxidation. Experiments have shown that in the 1,3-Bis ((trihydroxymethyl) methylamino) propane buffer system at pH 7.4, Cl ⁻ can accelerate the self oxidation rate of MbO ₂ by 2 times, while NO3⁻ inhibits this process.
Purification of glucose binding protein: When purifying the protein from the membrane of Sulfolobus solfataricus, BIS-Tris Propane can maintain protein activity and prevent precipitation or denaturation caused by pH changes. The protein activity recovery rate after purification can reach over 85%, significantly better than Tris buffer (60%).
2. Enzymatic research
1,3-Bis ((trihydroxymethyl) methylamino) propane shows outstanding performance in enzyme activity assays. For example, in alkaline phosphatase (ALP) activity detection, its buffer system can increase the enzyme reaction rate by 15% while reducing background interference. In addition, in lactate dehydrogenase (LDH) catalyzed reactions, 1,3-Bis ((trihydroxymethyl) methylamino) propane can stabilize enzyme conformation and extend enzyme half-life to twice that of traditional buffer solutions.
3. Electrophoresis experiment
In polyacrylamide gel electrophoresis (PAGE), 1,3-Bis ((trihydroxymethyl) methylamino) propane is used to maintain the pH stability of the electrophoresis buffer. Its advantages include:
Resolution improvement: In protein separation, the use of 1,3-Bis ((trihydroxymethyl) methylamino) propane buffer can increase band resolution by 20%, especially suitable for the separation of low molecular weight proteins (<20 kDa).
Reduce strip tailing: reduce strip tailing and improve quantitative accuracy by inhibiting the non-specific binding of protein and gel.
Cell culture: maintaining pH stability in the cell growth environment
1. Preparation of culture medium
1,3-Bis ((trihydroxymethyl) methylamino) propane can be used for pH regulation of cell culture media, especially for pH sensitive cell lines such as neurons and stem cells. For example, in neuronal culture, the use of 1,3-Bis ((trihydroxymethyl) methylamino) propane buffered medium can increase cell survival by 15% and promote synapse formation.
2. Serum free culture system
In serum-free culture medium, 1,3-Bis ((trihydroxymethyl) methylamino) propane can replace traditional buffering agents such as HEPES, providing a more stable pH environment. Experiments have shown that it can increase the proliferation rate of serum-free cultured cells by 10%, while reducing the apoptosis rate to below 5%.
1. Chromatographic analysis
In high-performance liquid chromatography (HPLC), 1,3-Bis ((trihydroxymethyl) methylamino) propane can be used as a mobile phase buffer to improve peak shape and enhance separation. For example, in amino acid separation, it can optimize the symmetry factor of the target peak to 0.9-1.1, significantly better than phosphate buffer solution (1.2-1.5).
2. Crystal growth
In protein crystal growth experiments, 1,3-Bis ((trihydroxymethyl) methylamino) propane can promote nucleation and control crystal size by adjusting the pH of the solution. For example, in the growth of lysozyme crystals, it can increase the uniformity of crystal size by 30% and reduce the defect rate to below 10%.
3. Metal ion chelation
The amino and hydroxyl groups in 1,3-Bis ((trihydroxymethyl) methylamino) propane molecules can form stable complexes with metal ions (such as Ca ² ⁺, Mg ² ⁺) to prevent precipitation. In solutions containing high concentrations of metal ions, it can increase the solubility of metal ions by 50% -80%, making it suitable for enzyme reaction systems that require the participation of metal ions.
There are two commonly used technical routes for the laboratory synthesis of BIS-Tris Propane. The following will provide a detailed introduction to the principles and specific steps of these two methods, while also referring to the relevant information in the article for explanation.
Method 1: Reaction method of ethylene glycol, ammonia water and formaldehyde
Principle:
This method is based on the chemical reaction between ethylene glycol, ammonia water, and formaldehyde. By controlling the reaction conditions and the proportion of reactants, the target product Bis Tris propane is ultimately generated.
Specific steps:
1. Preparation of raw materials: Prepare an appropriate amount of ethylene glycol, formaldehyde, and ammonia water.
2. Heating reaction: Add ethylene glycol and formaldehyde to the reaction vessel and heat to 70-90 ° C. This temperature range helps promote effective collisions and reactions between reactants.
3. Add ammonia solution: Slowly add ammonia solution while heating. The addition speed of ammonia should be moderate to avoid excessive reaction.
4. Temperature control: During the reaction, control the temperature between 90 and 100 ° C. This temperature range is conducive to the progress of the reaction while avoiding side reactions caused by excessively high temperatures.
5. Reaction completion and cooling: When the reaction reaches the predetermined time or when it is observed that the reactants have been completely consumed, stop heating and cool the mixture to room temperature.
6. Subsequent processing: Perform subsequent processing on the cooled mixture, such as filtration, drying, etc., to remove impurities and obtain pure Bis Tris propane.
Notes:
Throughout the entire reaction process, temperature, time, and the proportion of reactants should be strictly controlled to ensure the smooth progress of the reaction and the purity of the product.
Ammonia solution has a pungent odor and corrosiveness, and protective equipment should be worn during operation to ensure good ventilation in the laboratory.
Method 2: Method based on Chinese patent CN200810200543.2
Principle:
This method uses trimethylaminomethane and 1,3-dibromopropane as the main reaction materials, refluxes in ethanol solution for a certain time, undergoes acidification and alkalization treatment to obtain crude products, and then recrystallizes to obtain pure 1,3-bis (((trimethylolmethylamino) propane.
Specific steps:
1. Raw material preparation: Prepare an appropriate amount of trimethylaminomethane, 1,3-dibromopropane, and ethanol as the solvent.
2. Reflux reaction: Add the raw materials to an ethanol solution for reflux reaction. The reaction time should be adjusted according to the experimental conditions.
3. Acidification and alkalization treatment: After the reaction is completed, the mixture is subjected to acidification and alkalization treatment to remove impurities and adjust the pH value.
4. Recrystallization: Recrystallization of the processed mixture to further improve the purity of the product.
Advantages:
The raw materials used in this method are relatively common and easy to obtain.
The purity of the product can be effectively improved through steps such as recrystallization.
Notes:
During the reflux reaction process, the reaction temperature and time should be strictly controlled to ensure the smooth progress of the reaction.
During the process of acidification and alkalization, attention should be paid to adjusting the pH value to avoid adverse effects on the products.
In summary, the laboratory synthesis methods of BIS-Tris Propane mainly include the reaction method of ethylene glycol, ammonia water and formaldehyde, and the method based on Chinese patent CN200810200543.2. These two methods each have their own characteristics, and suitable methods can be selected for synthesis based on experimental conditions and requirements.
This product, scientifically named 1,3-Bis(hydroxymethyl)aminomethane (CAS 64431-96-5), is an important biological buffer. Its discovery is closely related to the development of 'Good' buffers in the mid-20th century. In 1966, N.E. Good and his colleagues proposed the concept of 'Good' buffers-these organic buffers minimally interfere with biological processes, have suitable pKa values, and are highly water-soluble, laying the foundation for their development.
Though its exact independent discovery year is not explicitly recorded, it emerged as a derivative of Tris buffer in the late 20th century, optimized to overcome limitations of traditional buffers. Initially, it was synthesized as a potential buffering agent, leveraging its unique molecular structure with dual amino groups and multiple hydroxyl groups to achieve a broad buffering range of pH 6.0-9.5.
In the early 2000s, researchers led by Mark Murrie began exploring its potential beyond buffering, discovering its role as a multidentate ligand in forming high-nuclearity metal complexes, which expanded its application scope. By the 2010s, its utility in biochemistry and molecular biology was fully recognized, being widely used in PCR, electrophoresis, and enzyme assays due to its low metal ion interference and high stability.
Today, it remains a staple reagent, its discovery and development reflecting the continuous pursuit of more reliable, versatile tools in life sciences and chemical research.
Selection Guide
BIS‑Tris propane is a versatile bio-buffer material, and in the customer's lab, they might be using it to prepare protein purification buffers, optimize enzymatic reaction systems, or adjust pH in cell culture or in vitro diagnostic reagents. No matter the use, what customers really care about is whether that small bottle of white crystalline powder can dissolve stably, whether it can consistently provide predictable buffering behavior, and whether they can get a clear, responsible, and evidence-based response in the shortest time if any issues arise. This is exactly the starting point of Bloomtechz's after-sales service.
Shipping the goods is just the beginning of the collaboration. Bloomtechz fully understands that overseas customers worry most about being left in the dark once the goods arrive, so they treat logistics tracking as the first commitment in their after-sales service. For every key step-booking, loading, departure, arrival at the destination port, and customs clearance-Bloomtechz proactively updates the customers instead of waiting for them to ask. Customers don't have to repeatedly log into shipping or train platforms to guess where their goods are because Bloomtechz clearly communicates every update.
Before and after the goods arrive, Bloomtechz also sends arrival notifications and bill of lading information in advance to help customers handle customs and pick up the shipment, all aimed at helping customers avoid unnecessary extra costs like detention or storage fees. In the procurement of biological reagents, timing is often closely linked to experimental plans. Whether a batch of buffer materials arrives on time can directly impact the progress of a research project, so Bloomtechz places great importance on transparent communication during shipment,
preferring to send multiple reminders rather than letting customers become anxious while waiting.
Once the goods arrive at the customer's warehouse, the second part of after-sales service kicks in. Bloomtechz includes a simple acceptance guide with the shipping documents, reminding the customer to check whether the marks, batch numbers, and documents match, inspect the outer packaging, and then complete the warehouse registration according to the guide. This guide isn't just a formality; it genuinely helps customers establish a proper receipt process and avoid disputes caused by oversight.
Even more important is the sample retention system. Bloomtechz keeps samples for every batch, with storage periods covering the contractually agreed warranty period. If customers later have doubts about the quality of a batch, both parties can use their retained samples for comparison testing, ensuring any quality disputes are based on objective data rather than opinions. Sample retention might seem simple, but it's the foundation of after-sales credibility, providing proof and reference for Bloomtechz's commitment to product quality.
If a customer notices any issues during use-be it appearance, solubility, clarity of solutions, or differences in some physicochemical indicators from the COA values-Bloomtechz has a clear and practical process in place. Response time is a non-negotiable commitment: after a quality issue is reported, customers usually receive a clear reply within 24 to 48 hours, after which verification and handling begin. If there's a disagreement over physicochemical indicators, either party can use the retained samples and, if needed, agree on a third-party testing organization to arbitrate based on objective test results.
If the problem is indeed with product quality, Bloomtechz will fulfill contractually agreed obligations for replacement, return, or refund, without evasion. If the issue arises from damage, moisture, or contamination during transport, Bloomtechz will use logistics records to assist in initiating insurance claims or arranging reshipment. The entire process aims to minimize customer loss, rely on facts and evidence, and ensures that customers know whom to contact, how to handle the problem, and how long it will take to resolve.
Frequently Asked Questions
How to control moisture drift?
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BIS-Tris Propane is highly hygroscopic, and regular drying often leads to moisture returning later. Bloomtechz uses sealed vacuum crystallization and inert gas packaging for drying, strictly controlling weight loss ≤1.0%. The entire production takes place in low-moisture cleanrooms, preventing crystals from absorbing water and clumping, ensuring buffer pH stability, and making it suitable for IVD and cell culture applications.
How do you control endotoxins within batches?
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Many manufacturers only label purity and ignore endotoxin and nuclease contamination. After purification, we add a pyrogen removal step, check endotoxins and DNase/RNase for each batch, and use non-metal-extracting equipment throughout the process to avoid micro heavy metal interference with downstream biochemical reactions. Batch testing charts are delivered alongside the COA.
Do you support particle size customization?
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The default crystal size is suitable for regular dissolution; for customers with automated dosing or continuous flow process needs, we can adjust recrystallization parameters to customize fine or large crystals, improve dissolution rate, reduce solution turbidity, and fit automated feeding equipment.
What's the moisture protection plan for shipping by sea?
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Although not a hazardous material, long-term sea transport in high humidity can cause moisture absorption. The product is sealed with argon-filled aluminum foil inside, combined with desiccants and corrugated protection, the container is prioritized as dry storage, avoiding long exposure on tropical routes. Each box comes with a humidity indicator card for customer inspection upon arrival.
How to ensure quality if the shipment stays at the port for a long time?
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Port delays can compromise packaging's moisture-proof performance. We reinforce export packaging with secondary sealing, pre-review SDS and classification documents to reduce the risk of inspections and detention. At the same time, we provide stability verification data showing that under compliant packaging, several weeks of room temperature delay does not significantly affect purity or moisture content.
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