Evans Blue CAS 314-13-6

Evans Blue, also known as Direct Blue 53, is an organic compound with the molecular formula C34H29N6NaO14S4 and CAS 314-13-6. It is a blue crystalline powder with a green metallic luster. In some descriptions, its color is described as dark brown or dark brown, which may be related to its purity, crystalline state, or observation conditions. Easy to dissolve in water, slightly soluble in ethanol, almost insoluble in organic solvents such as benzene, chloroform, and ether. Its solubility in water is high, reaching 280g/L at 20 ℃. There is a strong peak at the fluorescence wavelengths of 470nm and 540nm respectively, and a weak peak at 680nm. This characteristic makes it have potential application value in fluorescence labeling and detection. Has high toxicity. The LD50 value of its acute toxicity after intraperitoneal injection in mice is 340mg/kg (some data also provide 200mg/kg), indicating that it has certain harm to organisms. Therefore, special attention should be paid to safety during use and storage. Its high affinity with serum albumin makes it commonly used as a tracer in neuroscience research to observe the integrity of the blood-brain barrier (BBB). Mainly used for measuring blood solubility, and also for observing the integrity of the blood-brain barrier in neuroscience research. In addition, it can also serve as an L-glutamate uptake inhibitor and an AMPA/kainate receptor antagonist, with a wide range of biological activities.

Evans blue, as an organic compound, has multiple uses, mainly covering fields such as medicine, scientific research, and industry.

Measuring blood volume

One important use in the medical field is to measure blood volume. It forms high affinity complexes by binding with plasma albumin, enabling accurate measurement of plasma and blood volume. This method has broad clinical application value, especially in scenarios that require evaluating the patient's circulating blood volume, such as the diagnosis and treatment of diseases such as shock and heart failure.

Evaluate the integrity of the blood-brain barrier

Used to evaluate the integrity of the blood-brain barrier (BBB). Under normal circumstances, plasma albumin cannot penetrate the blood-brain barrier, so Direct Blue 53 bound to albumin cannot enter the nervous system. However, when the blood-brain barrier is damaged, it can cross the barrier and enter the nervous system, coloring it. By observing the staining of the nervous system, the integrity of the blood-brain barrier can be evaluated, which is of great significance for the diagnosis and treatment of neurological diseases.

Anti inflammation and anti itching

Has certain anti-inflammatory and antipruritic effects. In clinical practice, it is often used to treat inflammatory skin diseases such as contact dermatitis, eczema, and psoriasis, as well as skin itching symptoms caused by these diseases. By combining with proteins in the tissue to form complexes, it inhibits the stimulation of histamine and other allergens on the immune system, reduces the occurrence and development of inflammatory reactions, and thus achieves the goal of relieving local redness, swelling, and pain.

Stop bleeding and promote wound healing

In addition, it also has a hemostatic effect and can be used to prevent postoperative incision infections and promote postoperative wound healing. The hemostatic mechanism may be related to promoting platelet aggregation and blood coagulation, but the specific mechanism still needs further research.

Cell activity detection

 

It is often used as a tool for cell activity detection in the field of scientific research. Due to the efflux function of active cells, they cannot be stained with evans blue. Therefore, this method can be used to distinguish dead cells from live cells under a microscope. This method has broad application value in fields such as cell biology and pathology.

Study the permeability of the blood-brain barrier

 

As mentioned earlier, the high affinity for binding to serum albumin makes it of great value in studying blood-brain barrier permeability. By observing the distribution and staining of Direct Blue 53 in the nervous system, the permeability of the blood-brain barrier can be evaluated, providing important basis for the study of the pathogenesis and treatment plans of neurological diseases.

Drug carriers and tracers

 

It can also be used as a drug carrier or tracer. Due to its high affinity with serum albumin and good biocompatibility, Direct Blue 53 can carry drug molecules into the body and target specific tissues or cells. Meanwhile, its fluorescence characteristics also make it an ideal tracer for observing the distribution and metabolism of drugs in the body.

Although Direct Blue 53 has relatively few applications in the industrial field, as an organic dye, it still has the potential to play a role in certain industrial processes. For example, in the textile printing and dyeing industry, Direct Blue 53 may be used as a blue dye raw material; In chemical synthesis and pharmaceutical industry, it may also participate in the synthesis process of certain compounds as a raw material or intermediate. However, the specific situation and scale of these applications still require further investigation and understanding.

The organic synthesis methods of Everninomicin mainly include the McMurray nanosaccharide method and intramolecular glycation synthesis method. These methods involve constructing the polysaccharide ring structure and other functional groups of evans blue through complex organic synthesis routes.

• McMurray nanosaccharide method

The McMurray nanosaccharide method is a method of synthesizing Direct Blue 53 through saccharification and hydroxymethylation reactions, using hexahydroxymethylcyclohexyl sugar (MMC) as the starting material.

• Intramolecular glycation synthesis method

The intramolecular glycation synthesis method utilizes intramolecular glycation reactions to construct the polysaccharide ring structure of Direct Blue 53 through carbon chain elongation and cyclization reactions.

Evans blue powder, a vibrant and distinctive dye with a deep azure hue, has found extensive applications across various scientific and industrial domains. This synthetic dye, derived from a class of azo compounds, is noted for its high affinity for binding to serum albumin and other plasma proteins, making it an invaluable tool in a multitude of research and diagnostic procedures.

In medical research, it is employed to visualize and quantify vascular permeability and tissue injury. By intravenously administering the dye, researchers can observe its leakage into tissues, indicating sites of vascular damage or inflammation. This technique is particularly useful in studying conditions such as stroke, traumatic brain injury, and inflammatory diseases, providing crucial insights into the pathophysiology of these disorders.

Furthermore, it plays a pivotal role in the assessment of blood-brain barrier integrity. The dye's inability to cross an intact blood-brain barrier allows for the clear delineation of barrier disruptions, aiding in the understanding and treatment of neurological conditions.

Beyond medical applications, it is also utilized in dyeing fabrics, particularly those requiring a bold and enduring blue color. Its high color fastness and resistance to fading make it a favorite among textile manufacturers. Additionally, the dye finds use in staining biological tissues for microscopic examination, enhancing the visibility of cellular structures during histological analysis.

Safety considerations are paramount when handling it. While it is generally considered non-toxic, prolonged exposure or ingestion may cause irritation to the skin, eyes, and respiratory system. Therefore, appropriate protective gear, such as gloves, goggles, and masks, should be worn during handling and disposal.

Hot Tags: evans blue cas 314-13-6, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, 5 bromo 9 3 cyanophenyl 9H carbazole 3 carbonitrile, Additive, Solvent, Hinokitiol powder, Synthetic Chemical, 1 Butanesulfonic acid 1 1 2 2 3 3 4 4 4 nonafluoro benzo b naphtho 1 2 d furan 2 yl ester