DecaPeptide -12 is a synthetic polypeptide with rich donor activity, which can inhibit the differentiation and maturation of somatic cells, and is widely used in molecular biology research. The product has multiple application fields, such as immunology, neurophysiology, molecular biology, cell biology, pharmacology and other research fields.
DecaPeptide -12 is a complex protein polymer with high specificity and stability, and is easy to dissolve. Non-nutritive animal ingredients, liquid, colorless, transparent, odorless, pH 4-7 conformation. DecaPeptide-12 is a synthetic, whey protein-derived, non-cellular commandant analog with properties of controlled release, lipid binding, selective inhibition, natural peptide structure and inhibition of cell apoptosis. It is a polymer composed of 12 pancreatic peptide residues. It is synthesized by the secretion of insulin-like growth factor-1 (IGF-1) through the insulin-like peptide chain and the introduction of specific amino acid sequence through guanine molecule. In addition, DecaPeptide - 12 can also be synthesized by cell and other biological methods, such as in vitro puncture, serum antigen reaction or Escherichia coli can be produced in vitro at low cost.
DecaPeptide-12 uses:
1. DecaPeptide -12 is widely used in research fields around the world, such as immunology, neurophysiology, molecular biology, cell biology, pharmacology, and so on. It is a standard material for regulating cell characteristics and models.
2. DecaPeptide -12 can be used as a ligand to make polypeptide drugs have better biological activity and obtain better experimental results, which is an important means for DecaPeptide -12 to study the function of regulatory proteins.
3. DecaPeptide -12 can also be used as a vaccine carrier in the immune strategy, which can inhibit cell differentiation and cell maturation, improve the production rate of antibodies, and improve the immune effect of the vaccine.
4. In addition, DecaPeptide -12 can also be used for immune detection and diagnosis, immune protection and research on cell signal transduction mechanism.
5. It is also used in vaccine development, drug development and antigen determination in the pharmaceutical industry.
6. DecaPeptide -12 is a natural protein antigen, which is mainly used in the research and development of new drugs, vaccines, drug regulators, specific antigen identification and protein epitopes in the development of new drugs, as well as the analysis and research of the treatment of new viral infectious diseases. It can also be used for tumor detection and diagnosis of cysticercosis.
7. The chemical properties of DecaPeptide - 12 have been widely used. It has good stability, can be stored for several years at room temperature, and has high binding rate and specificity. In addition, it is also easy to dissolve. Before use, it can dissolve blood at room temperature under various pH conditions, and it is easy to use mRNA and cell surface to achieve immune response.
DecaPeptide -12 is now mainly used in Europe, the United States, Japan, China and other countries, and is actively expanding to other countries and regions around the world.
DecaPeptide-12 is a polypeptide composed of 12 amino acids. It can be used as a choice of antibody and can effectively inhibit the activation and expression of protein through antigen. The synthetic route of DecaPeptide-12 is divided into three main steps: construction of deoxyribonucleic acid (DNA), peptide synthesis and expression.
The construction of deoxyribonucleic acid is the synthesis process of DecaPeptide-12, which is divided into the following steps:
The first step is to use high-throughput sequencing technology to construct and verify the deoxyribonucleic acid (DNA) used to synthesize DecaPeptide-12. First, select a specific amino acid sequence as the template of amino acid sequence. Then, PCR technology is used to achieve high-throughput sequence synthesis, that is, to assemble several basic DNA units into DNA corresponding to precise amino acid sequences according to specific amino acid sequences. Finally, the cloned DNA was enriched using plasmid technology to ensure that specific amino acid sequences were cloned.
The second step is peptide synthesis. We need to realize peptide synthesis according to the newly constructed DNA. DNA is converted into polypeptides by PCR (polymerase chain reaction) and purified to eliminate other unwanted by-products. Commonly used transcriptases include T7 RNA polymerase, SP6 enzyme and Taq DNA polymerase, which can selectively convert different amino acid sequences.
Expression is the last step, which mainly uses Escherichia coli or other bacteria to express the purified polypeptide. Before expression, we need to effectively embed the polypeptide vector into Escherichia coli, so that the polypeptide can be effectively produced and accumulated.
DecaPeptide -12 (also known as dodecyl peptide), or DP-12 for short, is a polypeptide linked by 12 amino acids, which can help inhibit inflammation and improve immunity from various diseases.
The synthetic route of DecaPeptide-12 can be divided into four main steps:
1. Raw material preparation:
First, in order to synthesize DP-12, raw materials containing each amino acid need to be prepared. Amino acid is the basic component and the most important part of organic polypeptides. Therefore, high-quality and high-purity amino acids must be used when preparing raw materials. If the amino acid with poor quality is used, the quality and effect of the synthesis of DecaPeptide -12 will be affected.
2. Amino acid bonding:
Amino acid bonding is an extremely critical step in the production process of DecaPeptide -12, and also the longest and most difficult step. In this step, the prepared raw material containing each amino acid is combined with the other amino acid in order to form a peptide bond, that is, the addition reaction between the two ends of the amino acid generates an organic polypeptide.
3. Anhydrous synthesis:
After the completion of amino acid bonding, the peptide obtained may undergo cyclic addition reaction, and this is the best time for synthesis. Anhydrous synthesis can react in the polypeptide molecules bound by amino acids to form a complete DecaPeptide-12 molecule.
4. Purification treatment:
After the synthesis, the synthesized DecaPeptide -12 needs to be purified. DecaPeptide-12 molecule produced by anhydrous synthesis may also contain impurities, and purified DecaPeptide-12 molecule can be obtained after a series of purification treatment