Gentamycin Sulfate Powder CAS 1405-41-0

Gentamicin sand spores were placed on the slant medium for spore culture at 36 ℃ for 10 days. Gentamicin spores were obtained after slant culture;

The gentamicin spores obtained from the slant culture in step 1) are cultured in a shake flask. The access amount of the spores cultured in the shake flask is: 0.25cm2 of the spore slant is dug into a shake flask, the culture temperature is 36 ℃, the culture time is 42 hours, and the gentamicin shake flask mycelia is obtained;

Put the gentamicin shake flask mycelia obtained in step 2) on the seed culture medium for seed culture, and the seed culture input amount is: Gentamicin shake flask mycelia is injected into the shake flask seeds according to two thousandths of the volume of the seed culture medium, the culture temperature is 36 ℃, and the culture time is 48 hours to obtain gentamicin seed culture;

Put the gentamicin seed culture obtained in step 3) in the propagation tank for expanded culture. The gentamicin seed culture is obtained by adding 20% of the expanded medium volume, the culture temperature is 36 ℃, and the culture time is 42 hours;

Put the gentamicin propagation culture obtained in step 4) on the fermentation medium for fermentation. The amount of gentamicin propagation culture is 20% of the volume of the fermentation medium, the fermentation temperature is 34 ℃, and the fermentation time is 120 hours to obtain gentamicin fermentation products;

The gentamicin fermentation product obtained in step 5) is acidified to a pH value of 2 ‑ 3 to obtain gentamicin acidified solution; Acidizing treatment is conducted with hydrochloric acid with concentration of 30%, and adjust pH 2~3;

Neutralize the gentamicin acidizing solution obtained in step 6) to obtain gentamicin solution with pH value of 6.8; Neutralization treatment is carried out with 40% sodium hydroxide, and pH is adjusted to 6.8;

The gentamicin solution obtained in step 7) is treated with 732 resin to obtain the adsorbed gentamicin solution; The 732 resin used for adsorption treatment is 001 × 7 Strong acidic styrene cation exchange resin;

Remove Ca²⁺, Mg²⁺, Cl^‑ 1 from the adsorbed gentamicin solution obtained in step 8) on the column to obtain the ion removed gentamicin solution; The column for removing Ca²⁺, Mg²⁺and Cl^‑ 1 is Φ 1m high 2m column;

After passing the ion removed gentamicin solution obtained in step 9) through saturated resin, analyze it with ammonia water with a mass percentage of 4.5% to obtain gentamicin analytic solution; The saturated resin refers to the resin obtained by adsorbing 80000 units of gentamicin per milliliter of resin;

Decolorize the gentamicin solution obtained in step 10) with 711 resin to obtain gentamicin decolorization solution; 711 resin used for decolorization treatment is strongly basic styrene anion exchange resin;

Concentrate the gentamicin decoloring solution obtained in step 11) with a film to obtain gentamicin concentrated solution; The concentration amount is 500L/h, and the concentration is 1/7 of the original;

Add the concentrated gentamicin solution obtained in step 12) into sulfuric acid to prepare gentamicin sulfate; The concentration of sulfuric acid is 98%, adjusted to pH5.3;

The gentamicin sulfate obtained in step 13) is spray dried to obtain gentamicin sulfate finished product. The spray drying conditions are: inlet air temperature 130 ℃, outlet air temperature 85 ℃, and spray speed 100 L / h.