Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of hydroxynaphthol blue cas 63451-35-4 in China. Welcome to wholesale bulk high quality hydroxynaphthol blue cas 63451-35-4 for sale here from our factory. Good service and reasonable price are available.
Hydroxynaphthol blue, with the chemical formula C20H11N2Na3O11S3 and CAS number 63451-35-4, is an organic reagent widely used in fields such as biology and medicine. A solid that appears dark gray to purple at room temperature and pressure, with stable color that is not easily affected by light or air contact. It has good solubility in water, with a solubility of about 340 grams per liter. This characteristic makes its reaction and application in aqueous solutions more convenient and extensive. In addition, although the specific water solubility data may vary slightly due to changes in temperature, pressure, and other conditions, the overall solubility in water is strong. The pH value in aqueous solution is usually between 2-3 (measured at 20 ℃ with a 10g/l solution). This indicates that the compound exhibits a certain acidity in aqueous solution. As an important chemical reagent, it has demonstrated a wide range of physical applications in fields such as metal titration indicators, colorimetric reagents, visual pipeline closure detection, and biological research. Its unique color change characteristics and high sensitivity make it an important tool in many chemical reactions and substance detection.
|
|
|
|
Chemical Formula |
C20H11N2Na3O11S3 |
|
Exact Mass |
620 |
|
Molecular Weight |
620 |
|
m/z |
620 (100.0%), 621 (21.6%), 622 (13.6%), 623 (2.9%), 621 (2.4%), 622 (2.3%), 622 (2.2%) |
|
Elemental Analysis |
C, 38.72; H, 1.79; N, 4.52; Na, 11.12; O, 28.36; S, 15.50 |
Hydroxynaphthol Blue (HNB) is an organic compound that exists in the form of trisodium salt, with a molecular formula of C ₂₀ H ₁₁ N ₂ Na ∝ O ₁₁ S ∝ and a molecular weight of approximately 620.47. Its molecular structure contains naphthol derivatives and sulfonic acid groups, endowing it with good water solubility and metal complexation ability. As a metal indicator and biochemical reagent, HNB plays a key role in multiple fields. The following provides a detailed explanation of its core applications, technical principles, application scenarios, and operational points.
1. Detection of alkaline earth metal ions (calcium, magnesium, etc.)
HNB is a classic indicator for detecting alkaline earth metal ions, especially with high specificity for calcium ions (Ca ² ⁺). Its working principle is based on pH dependent color changes:
PH 7-12: The HNB aqueous solution appears blue and is not bound to metal ions at this time;
PH=10 and presence of Ca ² ⁺: HNB forms a complex with calcium ions, and the solution turns pink;
PH>13: The structure of HNB changes and the solution turns red.
Detection range: The concentration range of alkaline earth metal ions (such as calcium and magnesium) is 1-600 ppm, suitable for rapid analysis of environmental samples such as water samples and soil extracts. For example, in industrial water treatment, the titration method is used to determine the calcium ion content in water, and the endpoint color change (blue → pink) of HNB can accurately indicate the titration endpoint.
2. Rare earth metal ion detection (lanthanide elements)
HNB is also sensitive to rare earth metal ions such as lanthanum, cerium, neodymium, etc., with a detection range of 1-300 ppm.
The complex exhibits a significant characteristic peak at the maximum absorption wavelength of 650 nm, which can be quantitatively analyzed by spectrophotometry. For example, in rare earth mining, HNB is used to quickly screen the rare earth content in ore leachate and assist in process optimization.
3. Uranium ion (UO ₂² ⁺) detection
HNB is one of the few organic reagents that can directly detect uranium ions. In the nuclear industry, the formation of colored complexes between HNB and uranium ions can achieve rapid qualitative/quantitative detection of uranium in radioactive wastewater, with simple operation and low cost.
Molecular Biology: The 'Visual Signal Lamp' for LAMP Reaction
1. LAMP technology principle
Loop mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technique that rapidly amplifies target DNA under constant temperature conditions using specific primers. As a colorimetric indicator, HNB monitors the reaction process in real-time through color changes dependent on magnesium ions (Mg ² ⁺):
Initial state: HNB binds to Mg ² ⁺, and the reaction system appears violet colored;
Amplification proceeds: Mg ² ⁺ combines with the pyrophosphate ion (PPi ⁴⁻) produced by the reaction to form magnesium pyrophosphate precipitate. The HNB releases Mg ² ⁺, and the system gradually turns sky blue;
Unreacted system: Due to the non consumption of Mg ² ⁺, it maintains violet color.
2. Application scenarios
Pathogen detection: rapid screening of pathogens such as Shigella and COVID-19. For example, the LAMP detection kit based on HNB can complete sample detection within 30 minutes and directly judge the results through color changes (violet → sky blue) without the need for complex equipment, making it suitable for primary healthcare or on-site testing.
Gene expression analysis: By designing specific primers, HNB-LAMP can be used to detect gene mutations or changes in expression levels, such as quantitative analysis of cancer marker genes.
3. Key points of operation
Optimization of Mg ² ⁺ concentration: Hydroxynaphthol blue is necessary to accurately adjust the Mg ² ⁺ concentration in the reaction system (usually 2-8 mmol/L) to avoid false positives caused by excessive concentration or amplification efficiency affected by insufficient concentration.
PH control: The pH of the reaction system should be maintained at 7-9, deviation from this range may affect the color change sensitivity of HNB.
Temperature stability: LAMP reaction requires constant temperature (60-65 ℃), and temperature fluctuations may interfere with the binding/release process of HNB and Mg ².
1. Research on the interaction between proteins and nucleic acids
HNB can be used as a fluorescent probe to study protein conformational changes or nucleic acid hybridization kinetics through its binding/dissociation behavior with biomolecules. For example, in DNA protein interaction experiments, changes in the fluorescence intensity of HNB can reflect the occurrence of binding events.
2. Enzyme activity detection
Some enzymes (such as phosphatases) catalyze reactions that release phosphate ions, which bind to Mg ² ⁺ and affect the color of HNB.
By monitoring the rate of color change, enzyme activity can be indirectly measured. For example, in alkaline phosphatase (ALP) activity detection, the color change of the HNB system is positively correlated with enzyme activity.
3. Cell imaging and tracing
The fluorescence characteristics of HNB (maximum excitation wavelength of about 360 nm, emission wavelength of about 450 nm) make it suitable for dynamic monitoring of intracellular calcium ions. By observing HNB labeled cells under a fluorescence microscope, the calcium signaling process can be tracked in real-time.
1. Industrial water treatment
Calcium and magnesium ions are the main components causing scaling in industrial circulating water such as boiler water and cooling water. The HNB titration method can quickly determine the hardness (total calcium and magnesium content) of water, guide the dosage of water treatment agents (such as scale inhibitors), and prevent equipment corrosion or efficiency decline.
2. Environmental sample analysis
Soil heavy metal pollution detection: The combination of HNB and EDTA is used to determine the total amount of calcium and magnesium in soil extract by complexometric titration, and evaluate the degree of soil acidification or salinization.
Wastewater treatment monitoring: In industrial wastewater such as rare earth smelting and nuclear fuel cycle, HNB can quickly screen for pollutants such as uranium and rare earths, assisting environmental supervision.
The synthesis of hydroxynaphthol blue usually involves multiple steps, including pretreatment of starting materials, hydroxylation, sulfonation, azo and salt reactions. Here is a simplified synthesis path description:
Raw material selection: Choose appropriate naphthol derivatives as starting materials. For example, 2-naphthol or 1-naphthol can be chosen as the base compound.
Pre treatment: necessary purification and drying of the starting materials to ensure efficient reaction.
Objective: To introduce hydroxyl groups at specific positions in naphthols.
Reaction conditions: Usually require the presence of catalysts (such as transition metal catalysts, acidic or alkaline catalysts) and oxidants (such as hydrogen peroxide, potassium permanganate, etc.). The reaction temperature, pressure, and reaction time need to be adjusted according to the specific catalyst and raw materials.
Possible chemical equations (taking 2-naphthol as an example, assuming hydroxylation with hydrogen peroxide in the presence of a catalyst):
C10H8O+H2O2 → hydroxylation product+H2O
Note: The term 'hydroxylation product' here refers to a general term, and the actual product depends on the location and quantity of hydroxylation.
Objective: To introduce sulfonic acid groups onto hydroxylated products.
Reaction conditions: Concentrated sulfuric acid or sulfur trioxide is usually used as the sulfonating agent, and the reaction is carried out at low temperature to prevent excessive sulfonation. Neutralization and hydrolysis treatment are required after the reaction.
Possible chemical equations (taking hydroxylation products as an example):
Hydroxylation product+H2SO4 → Sulfonation product+H2O
Hydroxylation product+O3S → Sulfonation product+H2SO4
Note: The term 'sulfonated product' here is also a general term, and the actual product depends on the location and quantity of sulfonation.
Objective: To introduce azo groups onto sulfonated products through diazotization reaction.
Reaction conditions: Azo reagents (such as diazonium salts) and appropriate solvents are required. The reaction temperature needs to be controlled within a lower range to avoid the generation of by-products.
Possible chemical equations (taking sulfonation products and diazonium salts as examples):
Sulfonation products+diazonium salts → azo products+by-products
Note: The "azo product" here is a key part of the molecular structure of prpduct, but the specific structure depends on the structure of the sulfonated product and the conditions of the diazotization reaction.
Objective: To convert the diazotized product into the sodium salt or other metal salt form of product.
Reaction conditions: The diazonium product is reacted with metal hydroxides (such as sodium hydroxide) or carbonates to generate the corresponding salts. After the reaction, it needs to be filtered, washed, and dried.
Azo product+3ANaOH → C20H15N2NaO11S3+3H2O
Note: The "hydroxynaphthol blue trisodium salt" mentioned here is a common form of product, but the specific salt form may vary depending on experimental conditions and research objectives.
Hot Tags: hydroxynaphthol blue cas 63451-35-4, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, 11 bromospiro benzo c fluorene 7 9 xanthene , 9 bromonaphtho 1 2 b benzofuran, CAS 1955546 91 4, 9 9 dimethyl 7 4 4 5 5 tetramethyl 1 3 2 dioxaborolan 2 yl 9H fluorene 3 carbonitrile, CAS 1533406 38 0, 8H Dibenzo b b cyclopenta 2 1 e 4 3 g bisbenzofuran 6 10 diamine N6 N6 N10 N10 tetrakis 4 1 1 dimethylethyl phenyl 8 8 diphenyl