3,3'-Diaminobenzidine CAS 91-95-2
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3,3'-Diaminobenzidine CAS 91-95-2

3,3'-Diaminobenzidine CAS 91-95-2

Product Code: BM-2-5-227
CAS number: 91-95-2
Molecular formula: C12H14N4
Molecular weight: 214.27
EINECS number: 202-110-6
MDL No.: MFCD00007725
Hs code: 29214200
Enterprise standard: HPLC>999.5%, LC-MS
Main market: USA, Australia, Brazil, Japan, Germany, Indonesia, UK, New Zealand , Canada etc.
Manufacturer: BLOOM TECH Xi’an Factory
Technology service: R&D Dept.-1

Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of 3,3'-diaminobenzidine cas 91-95-2 in China. Welcome to wholesale bulk high quality 3,3'-diaminobenzidine cas 91-95-2 for sale here from our factory. Good service and reasonable price are available.

 

Selenium reagent is called DAB for short, and its scientific name is 3,3'-Diaminobenzidine. It forms a yellow stem with Se (W) in acidic medium. DAB is a kind of alkali, which darkens rapidly in the air. Its hydrochloride is a white needle, soluble in water, and its aqueous solution of hydrochloride can be used as a color developing solution. 3,3 '- diamino Benzidine (DAB) is widely used in chemistry, biochemistry, materials and other research fields.

 

At present, in countries with developed aerospace and military industries, DAB has begun to focus on research and is used as a monomer for the preparation of high-grade polymer materials, mainly for the synthesis of heat-resistant polymer resins and Synthetic fiber, but its application as a chromogenic agent in the field of Analytical chemistry is only seen in the photometric determination of selenium.

Produnct Introduction

3,3'-Diaminobenzidine CAS 91-95-2 | Shaanxi BLOOM Tech Co., Ltd

3,3'-Diaminobenzidine | Shaanxi BLOOM Tech Co., Ltd

Chemical Formula

C12H14N4

Exact Mass

214

Molecular Weight

214

m/z

214 (100.0%), 215 (13.0%), 215 (1.5%)

Elemental Analysis

C, 67.27; H, 6.59; N, 26.15

product-1-1

3,3'-Diaminobenzidine (DAB), with the chemical formula C ₁₂ H ₁₄ N ₄, is an important organic compound with unique chemical properties that make it widely used in various fields.

3,3'-Diaminobenzidine science | Shaanxi BLOOM Tech Co., Ltd

Materials Science Field

1. Preparation of High Temperature Resistant Resin and Fiber:
As a monomer, its application in the field of materials science is particularly prominent. The amino and benzene rings in its molecular structure endow it with good thermal and chemical stability, making it an ideal raw material for preparing high-temperature resistant resins and fibers. These materials can maintain good physical and chemical properties even in high temperature environments, and are therefore widely used in high-tech fields such as aerospace, automotive manufacturing, and electronics.

Aerospace field: The prepared high-temperature resistant resin can be used to manufacture key components such as aircraft engine parts and rocket casings. These components require stable mechanical and chemical properties under extreme high temperature conditions, and the application of DAB resin effectively improves the safety and reliability of aerospace vehicles.

In the field of automobile manufacturing, high temperature environments impose strict requirements on material properties in automobile engines and exhaust systems.

3,3'-Diaminobenzidine field | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine uses | Shaanxi BLOOM Tech Co., Ltd

The prepared high-temperature resistant fibers and resins can be used to manufacture components such as engine hoods and exhaust pipes, improving the high-temperature resistance and durability of automobiles.

In the field of electronic appliances: With the continuous improvement of the integration of electronic devices, the problem of heat dissipation is becoming increasingly prominent. The high-temperature resistant materials prepared by DAB can be used to manufacture components such as heat sinks and circuit boards, effectively improving the heat dissipation efficiency and service life of electronic devices.

2. Synthesis of Polymer Materials:
It can also be used to synthesize other types of polymer materials, such as polyamide, polyimide, etc. These materials have excellent mechanical properties, thermal stability, and chemical stability, and are widely used in industries such as machinery, chemical engineering, and instrumentation.
Polyamide material: As a monomer, it can react with other diacids or diamines to synthesize polyamide. Polyamide materials have the characteristics of high strength, high toughness, and good wear resistance, and are widely used in the manufacturing of fibers, plastics, coatings, and so on.

3,3'-Diaminobenzidine polymer | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine materials | Shaanxi BLOOM Tech Co., Ltd

Polyimide material: Polyimide is a high-performance engineering plastic with excellent high temperature resistance, mechanical properties, and chemical stability. As one of the monomers for synthesizing polyimide, it provides important raw materials for preparing high-performance polyimide materials. Polyimide materials have a wide range of applications in aerospace, electronics, automotive manufacturing, and other fields.

3. Preparation of slow-release flue gas denitrification agent:
A slow-release flue gas denitrification agent can be prepared by using 3,3'-diaminobenzidine as a precursor, synthesizing polybenzimidazole copolymer through UV irradiation and low-temperature catalysis, and then coating urea. This type of denitrification agent can slowly release denitrification active ingredients during flue gas treatment, improve denitrification efficiency, and reduce secondary pollution.
Improvement of denitrification efficiency: Slow release denitrification agents control the release rate of denitrification active ingredients to fully react with nitrogen oxides in flue gas, thereby improving denitrification efficiency.

3,3'-Diaminobenzidine gas | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine environment | Shaanxi BLOOM Tech Co., Ltd

Reduce secondary pollution: Traditional denitrification agents may generate secondary pollution during use, such as ammonia escape. The slow-release denitrification agent reduces the generation of secondary pollution by optimizing the denitrification process, which is of great significance for improving the quality of the atmospheric environment.
Reduced operating costs: The service life of slow-release denitrification agents is extended, reducing replacement frequency and operating costs, which has economic advantages for industrial flue gas treatment.

In the field of analytical chemistry

1. Determination of Selenium Content:
The application of DAB in the field of analytical chemistry is mainly based on its specific reaction with selenium element. Under acidic conditions, DAB reacts with tetravalent selenium to produce a yellow compound, which has a high absorbance at specific wavelengths and can be used to determine the selenium content in the sample by spectrophotometry.
High sensitivity: DAB spectrophotometry has high sensitivity and can detect low concentrations of selenium, meeting the needs of environmental monitoring, food safety testing, and other fields.

3,3'-Diaminobenzidine chemistry | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine reaction | Shaanxi BLOOM Tech Co., Ltd

Good selectivity: The reaction between DAB and selenium element has specificity, and other metal ions have less interference on the measurement results, which improves the accuracy of the measurement.
Widely applicable: DAB spectrophotometry can be used to determine the total selenium content in surface water, groundwater, domestic sewage, and industrial wastewater, providing accurate data for water quality assessment. Meanwhile, it can also be used for the determination of selenium content in samples such as food and soil.

2. As a color developer:
DAB can also be used as a chromogenic reagent for the determination of other metal ions. In certain specific chemical reaction systems, DAB can form colored complexes with certain metal ions. By measuring the absorbance or fluorescence intensity of the complexes, the content of metal ions in the sample can be indirectly determined.

3,3'-Diaminobenzidine color | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine metal | Shaanxi BLOOM Tech Co., Ltd

Metal element analysis: DAB colorimetric technology can be used to determine the content of metal ions such as copper, iron, and zinc, and has important application value in environmental monitoring, metallurgical analysis, and other fields.
Complex stability: The complex formed between DAB and metal ions has high stability, which is beneficial for the accuracy and repeatability of the determination.
Convenient method: DAB colorimetric technology is easy to operate, fast, and suitable for rapid determination of a large number of samples.

Biomedical field

1. Immunohistochemistry and Immunocytochemistry:
DAB is a commonly used chromogenic reagent in the field of life sciences, especially widely used in immunohistochemistry and immunocytochemistry. It is a commonly used chromogenic substrate for horseradish peroxidase (HRP), and in the presence of peroxides, oxidized DAB forms insoluble brown end products.
Tumor research: In tumor research, DAB colorimetric technology can be used to detect the expression of specific antigens in tumor tissues. Through immunohistochemical methods, the distribution and expression levels of specific antigens in tumor cells can be observed, providing important evidence for the diagnosis and treatment of tumors.

3,3'-Diaminobenzidine biomedical | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine diseases | Shaanxi BLOOM Tech Co., Ltd

Neuroscience research: DAB colorimetric technology can also be used for neuroscience research, such as detecting specific proteins or neurotransmitters in neurons. This helps to reveal the structure and function of the nervous system, providing new ideas for the treatment of neurological diseases.
Clear color rendering effect: The brown final product formed by DAB has a sharp contrast with the background, making it easy to observe and record. Meanwhile, DAB colorimetric technology has high sensitivity and specificity, and can accurately detect target antigens or proteins.

2. Preparation of biological probes
By chemically modifying DAB to bind with other biomolecules such as antibodies, nucleic acids, etc., biological probes with specific recognition functions can be prepared. These biological probes have important application value in biomedical research.
Molecular detection: DAB based biological probes can be used to detect specific molecules in biological samples, such as DNA, RNA, proteins, etc. This helps to reveal the structure and function of biomolecules, providing new methods for disease diagnosis and treatment.

3,3'-Diaminobenzidine probes | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine cell | Shaanxi BLOOM Tech Co., Ltd

Cell imaging: DAB biological probes can also be used for cell imaging research, by labeling specific cells or organelles to observe their distribution and dynamic changes within cells. This helps to reveal the laws of cellular life activities and provides powerful tools for cell biology research.
Potential application value: With the continuous development of biotechnology, DAB based biological probes have potential application value in drug development, gene therapy and other fields.

Other fields

1. Food additives (restricted use)
Although 3,3'-diaminobenzidine is toxic, it is also used as a food additive under certain conditions. This is mainly because it has a special aroma that can enhance the taste and flavor of food. However, due to its potential toxicity risks, its use is strictly restricted and regulated.
Usage restrictions: In most countries and regions, the use of DAB as a food additive requires strict safety assessment and approval procedures. The usage should be controlled within a safe range to ensure no harm to human health.

3,3'-Diaminobenzidine food | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine additives | Shaanxi BLOOM Tech Co., Ltd

Regulatory measures: Government departments strictly supervise the use of DAB as a food additive and regularly conduct safety assessments and monitoring. Once a safety hazard is discovered, immediate measures will be taken to prohibit or restrict its use.

2. Organic synthesis intermediates
DAB can also be used as an intermediate in organic synthesis to synthesize other organic compounds with special functions.

Synthesis of nitrogen-containing small molecules: Through the reaction of DAB with other compounds, nitrogen-containing small molecules with specific biological activities can be synthesized. These small molecules have potential application value in fields such as drug development and pesticide synthesis.

Synthesis of polymer compounds: DAB can also be used as a monomer or intermediate for synthesizing polymer compounds, and is used to prepare polymer materials with special properties. These materials have broad application prospects in fields such as materials science and electronics.

3,3'-Diaminobenzidine synthesis | Shaanxi BLOOM Tech Co., Ltd
3,3'-Diaminobenzidine field | Shaanxi BLOOM Tech Co., Ltd

3. Electrochemical field
DAB can be used to prepare modified electrodes through electrochemical polymerization, which have electrocatalytic effects on nitrite and hydrogen peroxide. This modified electrode has important application value in fields such as electrochemical sensors and electrochemical catalysis.
Electrochemical sensor: Electrochemical sensors based on DAB modified electrodes can be used to detect the concentration of nitrite ions in environmental samples.

Nitrite is a common water pollutant, and its concentration monitoring is of great significance for environmental protection.

Electrochemical catalysis: DAB modified electrodes have electrocatalytic effects on hydrogen peroxide and can be used for the decomposition reaction of hydrogen peroxide. This helps to develop efficient electrochemical catalysts and promote the development of electrochemical energy conversion and storage technologies.

3,3'-Diaminobenzidine pollutant | Shaanxi BLOOM Tech Co., Ltd

Manufacturing Information

3,3'-Diaminobenzidine Chemical | Shaanxi BLOOM Tech Co., Ltd

3,3'-Diaminobenzidine (DAB) is an important staining reagent widely used in biochemical and histological experiments. The following is the simplified synthesis route of DAB and the corresponding Chemical equation:

1. Preparation of raw materials:

-Phenol (or hydroquinone)

-Sodium hydroxide (NaOH)

-Hydrogen peroxide (H2O2)

2. Synthesis steps:

(1) Dissolve quinone in an appropriate amount of solvent and add an appropriate amount of NaOH solution.

(2) Add hydrogen peroxide to the solution and react for several hours to several days, keeping the temperature below 40 ° C.

(3) After the reaction is completed, solid-liquid separation is carried out through filtration or other methods.

(4) The solid product is washed and dried to obtain DAB.

3. Chemical equation:

The main steps involved in chemical reactions are as follows:

(1) 2C6H6O2+2NaOH → 3,3 '- Diphenylmethane dioxide+2H2O

(2) 3,3 '- Diphenylmethane dioxide+H2O2 → C12H14N4

FAQ
 

What is 3 3 Diaminobenzidine used for?

3,3′-Diaminobenzidine (DAB) is a widely used chromogen in histological staining methods and stained tissue is often used in downstream molecular analyses such as quantitative PCR (qPCR).

Is DAB stain toxic?

For decades, the rich brown stain produced by DAB has made it the chromogen of choice for IHC stains. However, precautions must be taken due to the fact that DAB is a known mutagen and potential carcinogen.

What is the full name of DAB?

3,3′-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to polybenzimidazole, which forms fibers that are renowned for their chemical and thermal stability.

 

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