Nystatin CAS 1400-61-9

Researchers carefully select high-yielding and genetically stable Streptomyces noursei strains from microbial libraries. These strains have undergone multiple rounds of breeding and purification to ensure their high-yield characteristics and genetic stability. This step is the foundation of fungal production, and only by selecting excellent strains can a solid foundation be laid for the subsequent production process.

In a sterile environment, researchers will inoculate dormant spores onto sterilized slant culture medium. By appropriate cultivation conditions such as temperature, humidity, and light, spores can germinate and grow sufficient mycelium. The purpose of this step is to obtain a sufficient number of active hyphae to provide sufficient inoculum material for subsequent seed cultivation.

Transfer the culture medium that has been activated and grown enough mycelium into the seed tank, and promote microbial proliferation through a step-by-step expansion cultivation method. During the seed cultivation process, it is necessary to strictly control the cultivation conditions, such as temperature, pH value, dissolved oxygen, etc., to ensure that microorganisms can grow normally and accumulate sufficient biomass. The purpose of this step is to obtain a sufficient number of active microbial cells to provide sufficient inoculum for subsequent large-scale fermentation.

This step is the core step in the production of nystatin. Inoculate the culture medium that has undergone seed cultivation and accumulated sufficient biomass into the fermentation tank. By controlling the fermentation conditions such as temperature, pH value, dissolved oxygen, stirring speed, etc., promote the growth of the strain and the synthesis of nysttin. The fermentation cycle is usually around 360-380 hours, during which regular sampling and testing of indicators such as the content and biomass of nysttin in the fermentation broth are required to monitor the fermentation process and make necessary adjustments.

The fermentation broth needs to be treated. Firstly, adjust the pH value of the fermentation broth to adapt to the subsequent extraction process. Then, impurities and microbial cells in the fermentation broth are removed through filtration, centrifugation, and other methods to obtain a relatively pure fermentation broth. The purpose of this step is to remove impurities that affect the subsequent extraction and refining processes, and improve the purity of the product.

Extraction and refining is one of the key steps in the production of antifungal agents. Solvent extraction and other methods are used to extract the fungal extract from the fermentation broth, and then high-purity fungal extract products are obtained through refining steps such as crystallization and drying. The purpose of this step is to remove residual impurities and solvents from the fermentation broth, and improve the purity and quality of the product.

Strict quality testing is conducted on the extracted and refined fungal extract products, including purity, potency, microbial limits, and other indicators. Only products that meet quality standards are allowed to be packaged and stored. During the packaging process, it is necessary to ensure the sealing and stability of the product to extend its shelf life and ensure its safety and effectiveness during use.