Papain Powder CAS 9001-73-4

Papain powder is an organic compound. White to light brown yellow amorphous powder or liquid, soluble in water, glycerin, insoluble in ethanol, chloroform and ether. It is a pure natural biological enzyme extracted from the white milk of the skin of the immature papaya fruit (Carica papaya) with modern bioengineering technology. Which derived from the latex of the papaya plant (Carica papaya), is a naturally occurring proteolytic enzyme renowned for its powerful digestive and healing properties. This enzyme belongs to the family of cysteine proteases, which are known for their ability to break down proteins into smaller peptides or amino acids.

It is a sulfhydryl (- SH) containing endopeptidase, with protease and esterase activities, and has a wide range of specificity. It has strong hydrolysis capacity for animal and plant proteins, polypeptides, esters, amides, etc. At the same time, it also has a synthesis function, and can synthesize protein hydrolysates into proteoids. Papain has the characteristics of high enzyme activity, strong thermal stability and safe and hygienic use.

Papain is a thiol protease extracted from papaya fruit. Its active center contains cysteine, which can specifically cleave specific amino acid residues in the protein peptide chain, breaking down large molecule proteins into small molecule peptides or amino acids. This characteristic makes it widely applicable in various fields such as food processing, pharmaceuticals, cosmetics, daily chemical products, feed, leather, textiles, etc.

Food Industry: From Tendering Meat to Improving Food Quality

 

1. Meat Tendering and Processing
It is the most widely used natural meat tenderizer in the food industry. It breaks down collagen and elastin in muscle fibers, destroys connective tissue structure, and makes meat soft and delicious. For example, in steak pretreatment, adding 0.05% -0.2% papain solution can significantly shorten cooking time and improve texture and tenderness; In the processing of meat products such as ham and sausage, it can improve the texture of the meat and prevent it from becoming hard during the processing. In addition, papain can also be used to extract non protein substances such as salmon skin oil, by degrading collagen in the skin and improving oil extraction efficiency.

 

2. Beer clarification and stability
During the beer brewing process, residual protein in the liquor may cause turbidity, affecting the appearance and stability of the product. By decomposing these turbid proteins, it helps beer maintain a clear and transparent appearance while extending its shelf life. Its effect is mild and will not affect the flavor of beer.
3. Improvement of baked goods
In baked goods such as bread and cakes, gluten in flour can be broken down to improve dough extensibility, resulting in a larger volume and softer texture of the baked product. For example, adding trace amounts of papain to bread can increase its specific volume by 10% -15% and result in a finer tissue structure.

 

4. Dairy processing
Hydrolyzable casein in dairy products can improve product taste and digestion absorption rate. For example, in cheese production, it can accelerate the coagulation process and shorten the production cycle; In yogurt processing, it can prevent whey precipitation and improve product stability.
5. Seasoning and health products
It can be used in soy sauce brewing, alcohol fermentation and other processes to improve nutritional value and flavor by decomposing protein in raw materials. In addition, it can also be used as an additive in health food to help digest and absorb protein, especially suitable for people with weak gastrointestinal function.

Medical field: from digestive assistance to trauma treatment

 

1. Treatment of digestive system diseases
As a digestive enzyme supplement, it can help break down difficult to digest proteins in the gastrointestinal tract, alleviate symptoms such as functional dyspepsia and chronic gastritis. Its pH range is wide, and it can still maintain partial activity in gastric acid environment. It is often combined with trypsin, amylase and other compound digestive enzyme preparations to improve digestive symptoms such as bloating and belching. For example, clinical studies have shown that taking compound preparations containing papain after meals can significantly improve protein digestibility and reduce gastrointestinal burden.

 

2. Trauma debridement and healing
It has the characteristic of selectively clearing necrotic tissue, which can decompose fibrin and necrotic cells in the wound without damaging healthy granulation tissue. In clinical practice, it is formulated as ointment or solution for burn wound scab removal, chronic ulcer debridement, etc. For example, in the treatment of diabetes foot ulcers, combined use of antibiotics can accelerate wound healing and reduce the risk of infection. Its mechanism of action is related to the degradation of inflammatory mediators bradykinin and the reduction of tissue edema.

3. Anti inflammation and pain relief
It can alleviate tissue edema and pain by regulating the release of inflammatory mediators.

 

Oral preparations are used as adjuvant therapy for postoperative swelling and traumatic edema; Topical application can alleviate redness and swelling caused by insect bites, arthritis, and other conditions. For example, clinical trials have shown that papain gel can significantly reduce the area of skin redness and itching after insect bites.
4. Other medical applications
It can also be used for blood type identification, insect bite treatment, gynecological disease adjuvant treatment, etc. For example, in blood type identification, it can hydrolyze antigens on the surface of red blood cells to help determine blood type; In the treatment of glaucoma, its anti-inflammatory properties can assist in reducing intraocular pressure.

Cosmetics and personal care: gentle cleansing and skin rejuvenation

 

1. Exfoliation and Whitening
It can decompose aging keratin in the epidermis, promote keratinocyte renewal without damaging newly formed cells. This characteristic makes it a core ingredient in acne and exfoliating skincare products. For example, the facial mask containing 2% -5% papain can reduce the skin roughness by 30% -40% and significantly improve the skin tone uniformity after 4 weeks of use. Its mildness is suitable for use on sensitive skin.

2. Anti aging and repair
By promoting collagen synthesis, improving skin elasticity, and reducing wrinkle formation.

 

In anti-aging skincare products, it is often compounded with ingredients such as vitamin C and hyaluronic acid to enhance the skincare effect. For example, clinical trials have shown that continuous use of eye cream containing papain for 6 months can reduce the depth of fine lines around the eyes by 20% -25%.
3. Cleaning and antibacterial
Can be added to daily chemical products such as soap and hand sanitizer to enhance stain removal power by decomposing protein stains; Meanwhile, its antibacterial properties can inhibit bacterial growth and ensure skin health. For example, hand sanitizers containing papain have an antibacterial rate of over 90% against Staphylococcus aureus.

The active extract of papaya lotion hydrolyzed protein is one of the earliest protease preparations. Fresh papaya milk is similar to milk, which can coagulate into gel in a few minutes. Papaya milk contains four kinds of proteinases, namely papain, papain A, chymosin B, and papain skin enzyme A. The common papain contains these four proteases.

The preparation of pure papain is to crystallize papain from the aqueous solution with ammonium sulfate and sodium chloride successively from fresh lotion and hydrochloric acid as activator. After that, Kimmel and Smith improved the method of Balals.

The method of KI mmel is to use hemisarcosine as the activator to obtain the water extract of crude enzyme from the dry milk, and then precipitate papain with ammonium sulfate and sodium chloride, and then crystallize in hemileucine solution. Six steps were needed for the complete purification, and the papain obtained accounted for 6-7% of the soluble components in papaya lotion.

Recently, affinity chromatography has been used to prepare papain with an interaction group of mio series, each protein molecule with sufficient activity, which is also prepared with dry lotion as raw material. The commercially available papain is the crude product of papain. Due to different preparation processes, its color can vary from milky white to reddish brown, and it contains a considerable amount of water insoluble substances.

Drying method is an important factor affecting the quality of crude papain. High quality products can be obtained by rapid drying of lotion at low temperature and spray drying. Freezing and drying can obtain fully soluble and sterile papain with special quality, which is competitive but expensive.

Papain powder is a cysteine containing proteolytic enzyme, and its proteolytic activity depends on the sulfhydryl group in the cysteine residue. Papain is a single chain protein composed of 212 amino acid residues. Each papain molecule contains seven cysteine residues, six of which form three disulfide bonds. Cysteine in the 25th position provides free active sulfhydryl groups. The tertiary structure of papain has been determined by x-ray crystallography. The imidazole ring of histidine 159 is very close to cysteine 25, and adheres to the back of the groove. Histidine residues at position 159 are partially embedded in the hydrophobic region formed by tryptophan and bound to aspartic ammonia at position 175 by hydrogen bond.

The fine, off-white to yellowish powder form of papain makes it highly versatile and easy to incorporate into various applications. In the culinary world, which acts as a tenderizing agent, enhancing the texture of meat by breaking down its tough protein fibers, making it more tender and flavorful. It's also used in marinades and meat tenderizers to expedite cooking times and improve overall palatability.

Beyond food, it finds extensive use in the health and beauty industry. Its gentle exfoliating action helps remove dead skin cells, promoting smoother, brighter skin. Its anti-inflammatory properties make it beneficial for treating minor skin irritations, wounds, and burns. Furthermore, it can assist in reducing inflammation and promoting healing in conditions like arthritis when applied topically or consumed as a supplement.

In the pharmaceutical sector, it is employed in the production of digestive aids and enzyme supplements, aiding in the management of digestive disorders. Its ability to break down mucus and phlegm also makes it a valuable addition to cough syrups and throat lozenges.

Overall, papain powder, with its natural, non-toxic, and multi-faceted benefits, stands as a testament to the wonders of nature's own chemistry, offering a holistic approach to wellness and beauty.

Hot Tags: papain powder cas 9001-73-4, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, 3-Phenyltoluene, 1 Methylpiperazine CAS 109 01 3, 1 4 Dibromobenzene 98 , 2 Phenylacetamide CAS 103 81 1, 2 6 Dichloropyrazine CAS 4774 14 5, Chlorocyclohexane CAS 542 18 7