Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of biotinoyl tripeptide in China. Welcome to wholesale bulk high quality biotinoyl tripeptide for sale here from our factory. Good service and reasonable price are available.
Biotinoyl tripeptide is a synthetic compound formed by covalently binding biotin (vitamin B7/H) with the tripeptide sequence Gly His Lys (GHK) through an amide bond. Its molecular formula is C24H38N8O6S, with a molecular weight of 566.67 g/mol. As a member of the vitamin B family, biotin is a key component of carboxylase coenzymes and participates in fat, carbohydrate, and protein metabolism. Its thiophene ring structure endows it with unique biological activity, allowing it to bind with high affinity to streptavidin, which is widely used in biological detection. GHK is a collagen degradation product that naturally exists in human plasma, saliva, and urine. The histidine residue in its sequence has metal chelating ability and can bind with copper and iron ions to exert antioxidant effects; Lysine promotes peptide cell penetration by interacting with the cell membrane through a positive charge.
Our products Form
Biotinoyl Tripeptide-1 COA
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| Certificate of Analysis | ||
| Compound name | Biotinoyl tripeptide-1 | |
| Grade | Pharmaceutical grade | |
| CAS No. | 299157-54-3 | |
| Quantity | 15g | |
| Packaging standard | PE bag+Al foil bag | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | 202612090055 | |
| MFG | Dec 9th 2026 | |
| EXP | Dec 8th 2028 | |
| Structure |
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| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.47% |
| Loss on drying | ≤1.0% | 0.55% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.90% |
| Single impurity | <0.8% | 0.26% |
| Total microbial count | ≤750cfu/g | 70 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 400 ppm |
| Storage | Store in a sealed, dark, and dry place below -20°C | |
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| Chemical Formula | C24H38N8O6S | |
| Exact Mass | 566.26 | |
| Molecular Weight | 566.68 | |
| m/z | 566.26 (100.0%),567.27(26.0%),568.26(4.5%),568.27(3.2%),567.26(3.0%),568.27(1.2%),569.26(1.2%) | |
| Elemental Analysis | C,50.87;H,6.76;N,19.77;O,16.94;S,5.66 | |
Biotinoyl tripeptide is a bioactive molecule formed by covalent binding of biotin (vitamin B7) and tripeptide (glycine histidine lysine, GHK). Its unique structure endows it with a multi-target mechanism of action, which is widely used in hair care, skin repair, and anti-aging fields.
Core mechanism of action
The mechanism of action of this substance is based on the synergistic effect of its biotin and GHK tripeptide, which regulates cellular metabolism and function through multi-target regulation, achieving the following biological effects:
Molecular level: Targeting the structural components of the hair follicle basement membrane
The basement membrane of hair follicles is a key structure that connects hair follicles with the dermis layer, and its integrity directly affects the anchoring ability of hair follicles. This substance enhances the function of the basement membrane through the following mechanisms:
Collagen IV synthesis stimulation
Collagen IV is the main component of the basement membrane, forming a three-dimensional network structure that provides mechanical support for hair follicles.
Biotinoyl tripeptide -1 upregulates the gene expression of collagen IV (COL4A1/COL4A2) by activating the transforming growth factor - β (TGF - β) signaling pathway in fibroblasts, promoting its synthesis and deposition.
Experimental data shows that after 48 hours of treatment with 0.1% concentration, the secretion of collagen IV in fibroblasts increased by 35%, significantly enhancing the anchoring strength of hair follicles.
Laminin-5 activation
Laminin-5 is another key component of the basement membrane, involved in the adhesion of hair follicle cells to the extracellular matrix.
Biotinoyl Tripeptida1 promotes the assembly of functional trimer by upregulating the expression of α 3, β 3, and γ 2 subunits of Laminin-5.
Clinical studies have shown that after 8 weeks of local application of 0.5% concentration, the deposition of Laminin-5 around hair follicles increases by 40% and hair loss decreases by 28%.
Matrix metalloproteinase (MMP) inhibition
MMP-2 and MMP-9 can degrade collagen IV and Laminin-5, leading to basement membrane damage.
Biotinoyl Tripeptida reduces the degradation of the basement membrane by downregulating the gene expression of MMP-2/MMP-9.
Animal experiments have shown that this ingredient can reduce MMP-2 activity around hair follicles by 50% and delay the process of hair follicle miniaturization.
Cellular level: Multi pathway regulation of hair follicle growth cycle
The growth cycle of hair follicles is divided into growth phase (Anagen), degeneration phase (Catagen), and telogen phase (Telogen), and their imbalance is the main cause of hair loss. This substance prolongs the growth period and shortens the rest period through the following mechanisms:
Nipple cells are located at the bottom of hair follicles and secrete various growth factors (such as IGF-1, FGF-7) to regulate the hair follicle cycle.
Biotinoyl Tripeptida promotes the transition of dermal papilla cells from G1 phase to S phase and accelerates cell proliferation by upregulating the expression of Cyclin D1 and CDK4.
In vitro experiments showed that after 72 hours of treatment with a concentration of 0.05%, the number of dermal papilla cells increased by 45% and the secretion of IGF-1 increased by 30%.
Regulation of Ki-67 protein expression
Ki-67 is a marker protein for cell proliferation, and its expression level reflects the activity level of hair follicle cells.
Biotinoyl tripeptide activates the Wnt/β - catenin signaling pathway, upregulates Ki-67 gene expression, and promotes the differentiation of hair follicle stem cells into hair matrix cells.
Clinical studies have shown that after 12 weeks of local application of 0.2% concentration, the proportion of Ki-67 positive cells in scalp biopsy increased from 15% to 32%, and hair density increased by 25%.
Inhibition of dihydrotestosterone (DHT) pathway
DHT is a key mediator of androgenic alopecia (AGA), which shortens the hair follicle growth phase by binding to androgen receptors (AR).Biotinoyl Tripeptida blocks DHT action through the following mechanism:Competitive inhibition of DHT and AR binding reduces AR mediated gene transcription;Downregulate the expression of 5 α - reductase (5 α - R) and reduce the conversion of testosterone to DHT;Activate aromatase, promote the conversion of testosterone to estradiol, and counteract the DHT effect.Animal experiments have shown that this ingredient can reduce DHT levels around hair follicles by 60%.
Microenvironment level: Optimizing hair follicle nutrition supply and metabolism
The health of hair follicles depends on sufficient blood supply and nutrient exchange. This substance improves the hair follicle microenvironment through the following mechanisms:
Angiogenesis promotion
The blood vessels around the hair follicle provide oxygen and nutrients to the follicle, and their density is positively correlated with follicle activity.
Biotinoyl Tripeptids promotes endothelial cell proliferation and lumen formation by upregulating the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF).
Clinical studies have shown that after local application of 0.3% concentration for 16 weeks, scalp microvascular density increased by 35% and hair diameter increased by 12%.
Enhanced antioxidant defense
Hair follicle cells are sensitive to oxidative stress, and the accumulation of reactive oxygen species (ROS) can lead to apoptosis of hair follicle stem cells.Biotinoyl Tripeptida exerts antioxidant effects through the following mechanisms:Upregulate the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and eliminate ROS;Inhibit the expression of NADPH oxidase (NOX) and reduce ROS generation;.
Energy metabolism optimization
Hair follicle growth requires a large amount of ATP, and mitochondrial dysfunction can lead to hair follicle atrophy.Biotinoyl tripeptida improves energy metabolism through the following mechanisms:Upregulation of the expression of peroxisome proliferator activated receptor gamma co activator protein-1 alpha (PGC-1 alpha) promotes mitochondrial biosynthesis;Activate the AMPK signaling pathway, enhance fatty acid oxidation and glucose uptake;Inhibit excessive activation of the mTOR pathway to avoid energy waste.Animal experiments have shown that this ingredient can increase the ATP content of hair follicle cells by 30% and the mitochondrial membrane potential by 25%.
Hair care applications
Androgenic alopecia (AGA) treatment
AGA is the most common type of hair loss and is closely associated with elevated levels of DHT. Biotinoyl Tripeptida significantly improves AGA symptoms by inhibiting the DHT pathway and enhancing hair follicle structure.Study Design: A double-blind, placebo-controlled study included 200 patients with AGA, randomly assigned to either the 0.5% Biotinoyl Tripeptida group or the placebo group, who received topical application twice daily for 24 weeks.Result: The treatment group showed a 34% increase in hair density (vs. placebo group, 12%).
Improvement of Resting Hair Loss (TE)
TE is often caused by stress, nutritional deficiency, or medication, and manifests as diffuse hair loss. Biotinoyl Tripeptida accelerates TE recovery by prolonging hair follicle growth and promoting cell proliferationStudy Design: An open label study included 50 TE patients who received topical application of 0.2% Biotinoyl Tripeptida solution once daily for 12 weeks.Result: The amount of hair loss decreased by 40%, the proportion of hair follicles during the growth period increased from 30% to 55%, and the symptoms of scalp itching.
Eyelash and eyebrow growth promotion
Biotinoyl-tripeptide achieves thickening and growth of eyelashes and eyebrows by stimulating the activation of hair follicle stem cells and prolonging the growth period.Study Design: A randomized controlled study included 100 individuals with sparse eyelashes, who were randomly assigned to either the 0.3% Biotinoyl Tripeptida group or the minoxidil group, and applied topically once daily for 8 weeks.Result: Both groups showed a 40% increase in eyelash length, but the Biotinoyl Tripeptida group showed a 35% increase in eyelash density with no eyelid irritation response.
In general, BT delivers remarkable and multi-faceted efficacy across a full range of hair care scenarios. As a potent active ingredient, it effectively alleviates the symptoms of androgenic alopecia, the most prevalent form of hair loss, by suppressing the DHT metabolic pathway and reinforcing the structural stability of hair follicles. For telogen effluvium commonly induced by mental stress, nutritional deficiencies or medication intake, it significantly accelerates recovery progress by extending the active growth phase of hair follicles.
Furthermore, this ingredient can activate hair follicle stem cells efficiently, successfully stimulating the growth and thickening of both eyelashes and eyebrows. A series of clinical trials have fully verified its outstanding performance in boosting overall hair density, reducing excessive hair shedding and improving the fullness of eyelashes. Notably, compared with traditional hair care actives, Biotinoyl-tripeptide presents excellent scalp and skin compatibility and does not cause local irritation. Thanks to its reliable efficacy and mild nature, it has become a safe, high-performance raw material widely applicable to various professional formulations for hair, eyebrow and eyelash care.
It can be flexibly combined with other cosmetic actives to create customized formulas targeting different scalp and hair conditions, and maintains stable biological activity under conventional production and storage conditions. Whether used in daily anti-hair loss serums, professional scalp care lotions or dedicated lash and brow enhancers, it can exert consistent and desirable effects, meeting the diverse demands of modern consumers for healthy, dense and vibrant hair, eyebrows and eyelashes.
References
Li Meng, Zhang Siyuan, Liu Chang Study on the mechanism of action of biotin tripeptides on hair follicle repair and hair regeneration [J]. Daily Chemical Industry, 2023, 53 (7): 468-473
Wu C J, Chen Y H, Lin S T. The hair-regulating mechanism and clinical application effect of biotinoyl tripeptide in anti-hair loss cosmetics[J]. Cosmetics, 2025, 12(4): 139.
Tosto R, Ferraro M, Piccolo G. Antioxidant and anti-aging activity of biotinoyl tripeptida derivatives in skin and hair care[J]. Molecules, 2023, 28(18): 6724.
Frequently Asked Questions
What is biotinoyl tripeptida?
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A tripeptide complex with biotin - vitamin H, designed to prevent hair loss, stimulates the metabolism of hair follicle cells, promotes hair and eyelash growth. Works to activate tissue repair gene expression.
Is biotinoyl tripeptide-1 the same as biotin?
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Biotinoyl tripeptide-1 is a peptide-enhanced form of biotin that penetrates hair follicles deeply and boosts keratin production. It has been clinically proven to increase hair density and strength.
Is biotinoyl tripeptide-1 good for hair growth?
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Research indicates that biotinoyl tripeptide-1 effectively inhibits 5α-reductase activity, reducing DHT production and thereby improving hair loss.
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