Leucine enkephalin is an endogenous pentapeptide composed of leucine, enkephalin, glycine, tyrosine, and serine. Molecular formula C28H37N5O7, CAS 58822-25-6. Structurally, as a pentapeptide, it has a relatively simple but compact molecular structure. Each amino acid residue is connected by peptide bonds to form a linear peptide chain structure. This structure gives product a relatively stable conformation, allowing it to perform its specific biological functions in living organisms. Meanwhile, due to the presence of various amino acid residues in its molecule, it exhibits a certain degree of structural diversity and complexity. Secondly, in terms of solubility, it usually has good water solubility. This is because its molecule contains hydrophilic amino acid residues such as glycine, tyrosine, and serine, which enable product to form a stable molecular structure in aqueous solution. May have other potential biological functions. For example, it may be involved in regulating memory and cognitive processes, and has a positive effect on improving problems such as memory decline and lack of concentration. In addition, it may also have antioxidant and anti-inflammatory effects, which are of great significance in protecting cells from damage and preventing the occurrence of diseases. It has multiple biological functions and plays an important role in neurological diseases, metabolic disorders, pain treatment, immune regulation, and neuroprotection. With in-depth research and understanding of the function of product, we are expected to develop more effective treatment methods for related diseases and make greater contributions to human health.
|
Customized Bottle Caps And Corks:
|
|
|
Chemical Formula |
C28H37N5O7 |
|
Exact Mass |
555 |
|
Molecular Weight |
556 |
|
m/z |
555 (100.0%), 556 (30.3%), 557 (2.7%), 556 (1.8%), 557 (1.7%), 557 (1.4%) |
|
Elemental Analysis |
C, 60.53; H, 6.71; N, 12.60; O, 20.16 |
Leucine Enkephalin is an important neuropeptide that is widely present in the central nervous system and has multiple biological functions. It interacts with the brain's μ,δ and κ The binding of three opioid receptors plays an important role in pain relief, sedation, and anticonvulsant effects, improving nervous system function, regulating metabolism, affecting appetite, regulating body temperature, and immune regulation.
1. Regulating nervous system function
Plays an important regulatory role in the nervous system. It can promote the growth, differentiation, and regeneration of neurons by regulating the release of neurotransmitters, thereby improving the function of the nervous system. This function enables leucine enkephali to play an important role in the treatment of neurological diseases such as Parkinson's disease and Alzheimer's disease. By regulating the function of the nervous system, leucine enkephali can improve symptoms such as motor disorders and memory loss in patients, and improve their quality of life.
2. Regulating metabolism
Has the function of regulating metabolism. It can promote the consumption of energy, regulate the metabolism of fat and sugar, so as to achieve the effect of regulating weight, improving obesity, diabetes and other metabolic diseases. This function makes leucine enkephali have potential application value in the treatment of metabolic diseases.
3. Analgesic and sedative effects
By binding to opioid receptors in the brain, it can exert analgesic and sedative effects. In situations of pain, stress, etc., the secretion of this substance will increase, thereby reducing pain and relieving tension. This function makes leucine enkephali have potential application prospects in pain treatment and the development of sedatives.
4. Affects appetite and temperature regulation
Can affect appetite and temperature regulation. It can help control weight by regulating the appetite center, affecting the production and inhibition of appetite. At the same time, it can also participate in the process of temperature regulation and maintain the stability of body temperature.
5. Immune regulatory function
In addition, it has immune regulatory functions. It can affect the activity and function of immune cells, regulate immune responses, and thus participate in the balance and regulation of the immune system. This function makes it have potential application value in the treatment of immune related diseases.
6. Neuroprotective effect
Has neuroprotective effects. It can improve cerebral blood circulation and metabolism, promote the repair and regeneration of brain tissue. This function makes leucine enkephalin play an important role in the treatment of neurological diseases such as brain injury and cerebral ischemia.
Leucineenkephalin is an important endogenous pentapeptide with multiple biological functions, particularly playing a crucial regulatory role in the nervous system. Due to its important physiological effects and potential application value, the synthesis method of leucine enkephali has always been a research hotspot in the fields of chemistry and biology.
The synthesis steps of leucine enkephalin:
1. Selection and pretreatment of solid-phase carriers
The commonly used carriers in solid-phase peptide synthesis include polystyrene resin, polyamide resin, etc. These carriers have good chemical stability and mechanical strength, which can effectively fix peptide chains and prevent them from falling off the support. When selecting a carrier, it is necessary to consider its characteristics such as pore size, specific surface area, and functional groups to ensure the smooth progress of the synthesis process.
2. Activation and condensation of amino acids
The activation of amino acids is one of the key steps in solid-phase peptide synthesis. Usually, amino acids react with activation reagents such as N-hydroxysuccinimide, pentafluorophenol, etc. to generate activated amino acid derivatives for condensation reaction with peptide chains on solid-phase carriers. The condensation reaction is usually carried out under alkaline conditions by adding an appropriate amount of condensation agent (such as triethylamine, N, N-dimethylformamide, etc.) to promote the reaction.
3. Extension and protection of peptide chains
On the basis of amino acid activation and condensation, gradually extending the peptide chain can be achieved by repeatedly adding new amino acid monomers and activation reagents. After adding each amino acid monomer, appropriate washing and drying steps are required to remove unreacted reagents and by-products.
4. Cutting and purification of peptide chains
When the peptide chain is extended to the target length, it needs to be cut off from the solid-phase carrier. This is usually achieved through acid hydrolysis or alkaline hydrolysis, depending on the properties of the carrier and peptide chain used. The cut peptide chain needs to be purified through appropriate steps to remove impurities and unreacted reagents. Common purification methods include gel filtration, ion exchange chromatography and high performance liquid chromatography.
Scope of sales channels
Leucine Enkephalin, as an important bioactive peptide, has broad application value in fields such as neuroscience, pharmacology, and biomedical research. The following is an analysis of its sales channel scope:
Professional biological reagent supplier
Renowned international and domestic suppliers
Internationally renowned biological reagent suppliers such as WAKO have a high reputation and extensive market coverage in the field of biological reagents. These suppliers typically have strict quality control systems and advanced production technologies, capable of providing high-purity, high-quality leucine enkephalin products. Its sales network covers the world, meeting the needs of customers in different regions by cooperating with distributors in various places or directly selling products to research institutions and enterprises. There are also many professional biological reagent suppliers in China, which have a deep foundation in the local market and a deeper understanding of domestic research needs and market environment. They can provide personalized services and more competitive prices based on the characteristics of domestic customers.
Peptide synthesis and production company
Some large peptide production enterprises, such as Jiangsu Jitai Peptide Industry Technology Co., Ltd., have strong production capacity and research and development capabilities. They can not only produce standard products such as leucine enkephalin on a large scale, but also customize synthesis according to customers' special needs. Small and medium-sized peptide synthesis companies are usually more flexible and able to quickly respond to customer order requirements. They focus on peptide synthesis technology and have certain technical advantages in some specific peptide product fields. These companies may primarily serve local or surrounding research institutions and enterprises, occupying a place in the market by providing personalized synthesis services and high-quality product quality.
Sales Area
Developed countries in Europe and America
Developed countries in Europe and America are at the forefront of biomedical research and pharmaceuticals, with a high demand for high-end biological reagents such as leucine enkephalin. These countries' research institutions and enterprises have invested a large amount of funds and manpower in neuroscience research, drug development, and have high requirements for product quality and performance. Internationally renowned biological reagent suppliers usually prioritize the European and American markets by establishing sales networks, participating in international exhibitions, and promoting and selling leucine enkephalin products. For example, research institutions and companies in countries such as the United States, the United Kingdom, and Germany are important international customers for leucine enkephalin.
EMERGING ASIA
Emerging economies in Asia, such as India, South Korea, Singapore, etc., have gradually increased their investment in biomedical research with the rapid development of their economies and the continuous improvement of their technological levels. The demand for biological reagents such as leucine enkephalin is showing a rapid growth trend among research institutions and enterprises in these countries. Some suppliers are starting to pay attention to these emerging markets, expanding their market share in Asian emerging economies by partnering with local distributors or setting up branch offices.
- In the mid-1970s, with the continuous advancement of separation and analysis techniques, scientists began to have the ability to isolate and identify trace amounts of bioactive substances from complex biological tissues. J. Hughes and other scientists decided to study pig brain because it contains abundant neuropeptides and is relatively easy to obtain. They designed a rigorous experimental protocol aimed at isolating substances with opioid like activity from pig brains.
- The research team first homogenized the pig brain, and then used a series of chromatographic separation technologies, such as ion exchange chromatography, gel filtration chromatography, to gradually separate the pig brain homogenate. In this process, they need to conduct rigorous activity testing for each separation step to ensure that the separated components have opioid like activity. After countless repeated experiments and optimized separation conditions, they finally isolated a small molecule peptide substance with opioid like activity from pig brain.
- After successfully isolating substances with opioid like activity, the research team used advanced technologies such as mass spectrometry and amino acid sequence analysis to identify the structure of the substance. After detailed analysis, they determined the amino acid sequence of this substance as Tyr Gly Gly Phe Leu and named it Leucine Enkephalin. This discovery was officially announced in 1975, marking the birth of leucine enkephalin.
Leucine enkephalin exemplifies the intricate interplay between endogenous peptides and physiological processes. Its dual role in pain relief and emotional regulation underscores its therapeutic potential, while challenges in pharmacokinetics and receptor dynamics highlight the need for innovative drug design. As research progresses, Leu-ENK and its derivatives may redefine pain management and offer novel treatments for neurological and cardiovascular disorders. The future of opioid therapy lies in harnessing the body's own mechanisms, and Leu-ENK stands at the forefront of this paradigm shift.
Hot Tags: leucine enkephalin cas 58822-25-6, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, Bremelanotide Powder CAS 189691 06 3, CJC 1295 Peptide, Epitalon Powder CAS 307297 39 8, Best Growth Hormone Releasing Peptide CAS 87616 84 0, LONG R3 IGF I CAS 143045 27 6, Selank Peptide CAS 129954 34 3