Shaanxi BLOOM Tech Co., Ltd. is one of the most experienced manufacturers and suppliers of walnut oligopeptide in China. Welcome to wholesale bulk high quality walnut oligopeptide for sale here from our factory. Good service and reasonable price are available.
Walnut oligopeptide is a small-molecule bioactive peptide substance refined from defatted walnut meal of natural walnut kernels through protein extraction, targeted enzymatic hydrolysis with compound biological enzymes, membrane separation and purification. Compared with conventional walnut protein, the peptide can be rapidly absorbed and utilized by the human body directly without gastrointestinal decomposition, while retaining the original neurotrophic, antioxidant and lipid-regulating active components of walnuts.
Our Products Form
Walnut Oligopeptide COA
 |
||
| Certificate of Analysis | ||
| Compound name | Walnut Oligopeptide | |
| Grade | Pharmaceutical grade | |
| Quantity | 20g | |
| Packaging standard | PE bag+Al foil bag | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | 202601090056 | |
| MFG | Jan 9th 2026 | |
| EXP | Jan 8th 2029 | |
| Structure | N\A | |
| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.33% |
| Loss on drying | ≤1.0% | 0.19% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.80% |
| Single impurity | <0.8% | 0.67% |
| Total microbial count | ≤750cfu/g | 445 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 670ppm |
| Storage | Store in a sealed, dark, and dry place below 2-8°C | |
|
|
||
Functional Foods and Nutritional Supplements
Widely applied in the functional food industry, walnut oligopeptide features small molecular weight and easy absorbability, making it an efficient nutritional supplement ideal for people with weak digestive functions (the elderly and postoperative patients) and rapid energy supplementation scenarios. In sports nutrition, it promotes muscle repair, relieves exercise fatigue and accelerates physical fitness recovery. In elderly nutrition, it ameliorates muscle loss, enhances immunity and supports healthy aging. In children's nutrition, it assists brain development and improves learning and memory ability.
As a nutritional fortifier, it applied in fermented foods (soy sauce, fermented soybean), it serves as a nitrogen source to boost microbial growth, shorten fermentation cycles, and enhance product flavor and nutritional value. Additionally, it can be formulated into solid beverages, oral liquids, chewable tablets and other dosage forms to meet the convenient and efficient nutritional supplementation needs of different groups.
Pharmaceutical and Health Care Fields
Neuroprotection and Cognitive Improvement
Rich in amino acids including glutamic acid, the peptide can increase acetylcholine content in the brain, accelerate cerebral tissue metabolism and restore brain cell function, exerting remarkable effects on alleviating brain fatigue, improving memory and preventing Alzheimer's disease. Studies have shown that it inhibits the activities of nerve-related enzymes such as BACE1 and AChE, reduces beta-amyloid deposition, protects nerve cells and delays the progression of neurodegenerative diseases. Animal experiments indicate that appropriate intake of the peptide can elevate the level of neurotransmitters related to memory transmission in mice and enhance learning and memory capacity.
Antioxidation and Anti-Aging
It exhibits strong antioxidant activity, capable of scavenging free radicals in the body, reducing oxidative stress damage and delaying cellular senescence. It has been verified that its DPPH free radical scavenging capacity and Fe²⁺ chelating activity are superior to glutathione (GSH) at the same concentration. It effectively maintains cell membrane integrity, alleviates lipid peroxidation and reduces the risk of aging-related diseases. In skin health care, it promotes collagen synthesis, enhances skin elasticity, improves skin texture and reduces wrinkles, and is widely used as a raw material in anti-aging health products and skin care formulations.
Cardiovascular Health Regulation
The peptide inhibits angiotensin-converting enzyme (ACE) activity, relieves vasoconstriction, reduces peripheral vascular resistance and assists in blood pressure regulation. Research from the School of Food Science and Technology, Jiangnan University shows its ACE inhibition rate reaches 72.3%, presenting a significant auxiliary antihypertensive effect. Meanwhile, it modulates lipid metabolism, reduces serum total cholesterol and triglyceride levels, elevates high-density lipoprotein cholesterol, lowers the risk of atherosclerosis and protects cardiovascular health.
Digestive Function Regulation
It regulates the secretion of gastric acid and pepsin, promotes the release of gastrointestinal hormones (motilin, substance P, gastrin), enhances gastrointestinal peristalsis and improves digestive function. Animal experiments demonstrate that it increases the output of free and total gastric acid, reduces pepsin activity, accelerates small intestinal peristalsis, and relieves indigestion and constipation, making it suitable for people with gastrointestinal dysfunction.
Anti-Hypoxia and Immune Modulation
Walnut oligopeptide has prominent anti-hypoxia activity, which can prolong the survival time of mice under normal-pressure hypoxia, sodium nitrite poisoning and acute cerebral ischemia, and improve the body's tolerance to hypoxic environments. It is applicable to plateau operations and mental fatigue scenarios. Moreover, it activates immune cell activity, stimulates the secretion of immune factors, enhances the body's non-specific immune function, strengthens physical resistance and prevents infectious diseases.
Cosmetics and Daily Chemical Products
With excellent water solubility, stability, biocompatibility and skin repair activity, it is widely used in cosmetics and daily chemical products. In skin care, it facilitates skin cell regeneration, repairs damaged skin, strengthens the skin barrier, moisturizes and locks in water, brightens dull complexion, and fades pigmentation and wrinkles. It is commonly added to facial masks, essences, repair lotions and face creams.
In hair care, it nourishes the scalp, optimizes scalp microecology, promotes hair growth, enhances hair toughness and reduces hair loss and breakage, and is applied in shampoos, conditioners and hair masks. Furthermore, it can be used as a natural antioxidant, humectant and emulsifier in facial cleansers, body washes, massage creams and other daily chemical products. Mild and non-irritating, it is suitable for sensitive skin.
Other Applications
Animal Nutrition and Feed Additives
It improves feed utilization rate, promotes growth and development, enhances immunity and optimizes meat quality of livestock and poultry (pigs, chickens, cattle), aquatic products (fish, shrimp, crabs) and pet food. In pet food, it boosts digestion and absorption, improves physical fitness and promotes skin and coat health, and is used in pet dry food, snacks and canned food.
Biological Fermentation and Industrial Applications
It can be used as a nutritional component of biological fermentation culture medium, providing a nitrogen source for microbial growth, facilitating the synthesis of fermented products such as enzyme preparations, antibiotics and amino acids, and improving fermentation efficiency and product quality. In addition, due to its amphiphilic structure, it can serve as an intermediate for surfactants and emulsifiers in the industrial production of cosmetics and detergents.
References
Xinghui Tianli (Hangzhou) Pharmaceutical Co., Ltd. A Walnut Oligopeptide, Its Preparation Process and Application: China Patent CN105936927A, 2016-09-14.
Kunming University of Science and Technology, Yunnan Academy of Forestry and Grassland Sciences. Walnut Polypeptide, Its Preparation Method and Application: China Patent CN119823216A, 2025-04-15.
Li Y, et al. Anti-hypoxia effects of walnut oligopeptides (Juglans regia L.) in mice[J]. Food & Function, 2021, 12(15): 7245-7253.
Raw Material Pretreatment
High-quality walnut kernels are selected as raw materials, mechanically shelled, cleaned to remove impurities, and crushed to 40~60 mesh. Defatting is carried out via physical pressing or solvent extraction to obtain defatted walnut residue, which is then crushed to 45 mesh for later use.
Walnut Protein Extraction
Deionized water is added at a solid-liquid ratio of 1:8~1:12 (w/v). The pH value is adjusted to 8.5~9.0 with sodium bicarbonate or sodium hydroxide solution, followed by soaking at 55~65℃ for 2~4 hours. The mixture is centrifuged at 3000~5000×g for 15~20 minutes to remove precipitates, and the supernatant is collected. The pH is adjusted to 4.5 with citric acid or glacial acetic acid. After standing for 2.5 hours, centrifugation is performed again. The lower precipitate is washed with water until neutral to obtain walnut protein extract.
Compound Enzymatic Hydrolysis (Core Process)
A two-step dual-enzyme hydrolysis method is adopted: first, neutral protease (6000 u/g, accounting for 0.5%~2.0% of protein mass) is added for hydrolysis at pH 7.0 and 45℃ for 3 hours. After enzyme inactivation, alkaline protease (6000 u/g) is added for hydrolysis at pH 9.1 and 51℃ for 3.7 hours. Temperature, pH value and time are strictly controlled during enzymatic hydrolysis to ensure the hydrolysis degree exceeds 34%, yielding oligopeptides with molecular weight below 800 Da.
Filtration, Purification and Concentration
The enzymatic hydrolysate is filtered through an 80~100 mesh screen and centrifuged to remove impurities. Membrane separation technology (ultrafiltration/nanofiltration) is applied for purification to eliminate macromolecular proteins, polysaccharides and other impurities and enrich oligopeptides. Vacuum concentration is performed at 60~70℃ under -0.08~-0.1 MPa until the solid content reaches 20%~30%.
Drying and Finished Product Preparation
The concentrated liquid is processed by spray drying (inlet air temperature: 180~200℃, outlet air temperature: 80~90℃) or freeze drying to obtain off-white to light yellow powdered walnut oligopeptide. The product has a peptide content ≥70%, moisture ≤8%, and molecular weight mainly distributed in 200~800 Da.
References
Xinghui Tianli (Hangzhou) Pharmaceutical Co., Ltd. A High-Purity Walnut Oligopeptide and Its Industrial Preparation Method and Application: China Patent CN107446975B, 2020-06-16.
Technology Transfer Center of Wuhan Polytechnic University. Separation Protein and Polypeptide Preparation Technology from Cold-Pressed Walnut Cake[EB/OL]. 2025-12-15.
Cautions
Although the peptide features high safety and good tolerance, standardized consumption is still required to avoid potential risks. It is strictly prohibited for individuals allergic to walnuts or nuts, as it may cause rashes, itching and even anaphylactic shock.
Infants, patients with severe renal insufficiency, acute gastrointestinal ulcers and pancreatitis are not recommended to take it without professional guidance, since peptide metabolism may increase renal burden or irritate damaged mucous membranes.
References
Anhui Shengmeinuo Biotechnology Co., Ltd. Enterprise Food Safety Standard Q/ASMN0016S-2024 Walnut Protein Peptide (Walnut Peptide)[S]. 2024-08-23.
Hot Tags: walnut oligopeptide, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, Tesamorelin CAS 218949 48 5, DecaPeptide 12 CAS 137665 91 9, Bremelanotide Powder CAS 189691 06 3, Best Growth Hormone Releasing Peptide CAS 87616 84 0, Kisspeptin powder, Lpamorelin Powder CAS 170851 70 4