Phloroglucinol powder, also known as 1,3,5-trihydroxybenzene, is a versatile organic compound belonging to the phenol family. This fine, white to light brown powder exhibits unique chemical properties that make it a key ingredient in various industrial and research applications.
Its primary characteristic lies in its ability to form strong hydrogen bonds, allowing it to serve as a starting material for the synthesis of numerous complex molecules. In the pharmaceutical industry, it serves as a precursor for the production of certain drugs, owing to its reactivity and structural versatility.
Moreover, it finds application in the dye industry, where it contributes to the development of colorants with enhanced stability and fastness. Its use in analytical chemistry is equally significant, as it serves as a reagent for detecting the presence of certain functional groups in organic compounds.
In addition, it displays antibacterial and antifungal properties, making it a potential candidate for natural preservatives in food and cosmetics. Researchers are also exploring its potential in the field of nanotechnology, where its unique properties could lead to the development of innovative materials.
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Chemical Formula |
C6H6O3 |
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Exact Mass |
126.03 |
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Molecular Weight |
126.11 |
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m/z |
126.03 (100.0%), 127.04 (6.5%) |
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Elemental Analysis |
C, 57.14; H, 4.80; O, 38.06 |
biocatalytic synthesis
Dilute the cell to a certain concentration, then take the bacterial solution and add it to the mixed solution of NaNO2 solution and HAC-NaAC buffer solution, and culture it by shaking in a water bath at about 37 ℃. Add them into Na2HPO4 buffer solution respectively, mix them well, smear them on the plate containing LiCl, and cultivate them in dark at about 37 ℃.
The genomic DNA of bacteria Pseudomonas fluorescens Pf-5 was extracted, and the polyketone synthase gene (phlD) was obtained by PCR amplification; The mutant gene phlD1 was obtained by site directed mutagenesis. The target fragment was recovered by gel recovery kit. The target fragment was digested by the corresponding restriction enzyme at 37 ℃ for 3-4 hours. The target fragment was purified by ethanol precipitation; The prokaryotic expression vector pET-30a (+) was digested with the same restriction enzyme, and the gel recovery kit was used for recovery and purification; The prokaryotic expression vector pET-phlD1 was constructed when the ratio of the vector to the exogenous fragment was 1:3-10, and it was connected overnight at about 16 ℃; The constructed recombinant plasmid was transformed into anti phloroglucinol Escherichia coli by heat shock at 42 ℃, and the positive recombinant strain was obtained through enzyme digestion identification, PCR identification and sequencing screening; ‑ The strain was stored at about 80 ℃.
Inoculate the engineered bacteria into LB liquid culture medium (including 30-50μG·mL -1 kanamycin), when the OD600 is about 0.4-0.6 at 37 ℃, the inducer IPTG is added to the bacterial solution, and then the target protein is induced to express at 37 ℃ for 3 ‑ 5 hours; Take out the induced culture solution, take the sediment and wash it with phosphate buffer, and then suspend the cells with this buffer at a ratio of 1:10 (w/v). Add 2×SDS-PAGE loading buffer, boiling, SDS-PAGE electrophoresis detection, the corresponding fusion protein bands can be detected.
Fermentation of phloroglucinol Prepare fermentation medium, sterilize, and conduct aseptic inoculation when cooling. According to the optimization results of orthogonal experiment, the initial culture medium, through the induction of temperature electrode and pH electrode, automatically adjust the temperature and pH of fermentation liquid, and stir the fermentation culture.
Isophloroglucinol from the fermentation supernatant was extracted by ethyl acetate, then ethyl acetate was recovered and concentrated under reduced pressure to obtain phloroglucinol.
As a Biological Reagent
- Biological Research: Widely used in biological research, particularly in plant biology and biochemistry. It serves as a valuable tool for scientists studying plant metabolism, particularly in the analysis of lignin, a complex organic polymer found in plant cell walls.
- Assay Reagent: It is also used as a reagent in assays to detect and quantify compounds such as vanillin and lignin, making it an essential component in the characterization of plant cell wall components.
In Dye Industry
- Dye Synthesis: In the synthesis of dyes, particularly those used in histological staining of plant tissues. Its unique chemical properties allow for the production of dyes that can effectively stain specific plant cell structures, facilitating microscopic analysis.
Chemical Analysis
- Analytical Reagent: As a chemical compound, it serves as an analytical reagent in various chemical analyses. It is used to determine the presence and concentration of compounds like sugar aldehydes and polysaccharides, which are essential for understanding the chemical composition of various samples.
Pharmaceutical Industry
- Pharmaceutical Research: Although its primary applications are in the scientific realm, it has also shown promise in pharmaceutical research. It is being investigated for its potential use in the development of new therapeutic agents, particularly in the area of smooth muscle relaxants.
- Smooth Muscle Relaxant: Notably, Phloroglucinol is a non-atropine, non-narcotic smooth muscle relaxant that does not produce anticholinergic side effects. This makes it an attractive candidate for the treatment of conditions caused by smooth muscle spasms, such as those found in the gastrointestinal and genitourinary systems.
Other Applications
Furthermore, the antibacterial and antifungal characteristics of phloroglucinol powder suggest its promising applications as a natural preservative in various industries, including food and cosmetics. These properties are particularly attractive due to the growing consumer demand for natural and safer alternatives to synthetic preservatives.
In the food industry, it could help extend the shelf life of perishable products, reducing food waste and improving food safety. Its ability to inhibit the growth of harmful bacteria and fungi makes it a valuable addition to food preservation strategies.
Similarly, in the cosmetics industry, it could serve as a natural preservative, protecting products from microbial contamination and enhancing their overall safety for consumers. This could be particularly beneficial for brands that prioritize natural and organic ingredients in their formulations.
Beyond its use as a preservative, researchers are also intrigued by the potential of it in the field of nanotechnology. Its unique physical and chemical properties make it a promising candidate for the development of innovative materials with novel functionalities. For instance, nanoparticles derived from phloroglucinol could be used in drug delivery systems, where they could help transport therapeutic agents to specific targets within the body, enhancing treatment efficacy and reducing side effects.
In conclusion, the multifaceted nature of phloroglucinol powder, combined with its natural origin and broad range of properties, positions it as a promising material for various applications, including as a natural preservative in food and cosmetics, and as a building block for innovative nanomaterials. Further research is needed to fully unlock its potential and explore new avenues for its use.
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