cjc 1295 Peptide, CAS 863288-34-0. Usually white or similar white powder, amorphous or fluffy, easy to dissolve in polar solvents such as water, methanol, ethanol, but difficult to dissolve in non-polar solvents such as chloroform, dichloromethane, etc. At room temperature, CJC1295 has high stability and can be stored and transported under dry, cool and dark conditions, and the validity period is generally 2-3 years. CJC-1295 is a polypeptide GHRH (growth hormone releasing hormone) compound, which can promote the release of growth hormone (HGH) secreted by the pituitary gland, and thus has been widely used in many clinical and non-clinical fields.
Regarding CJC 1295, we have 3 webpages to introduce and describe the product in detail according to different ingredients, namely:
CJC 1295 With DAC : https://www.bloomtechz.com/synthetic-chemical/peptide/cjc-1295-with-dac-cas-863288-34-0.html
CJC 1295 Peptide : https://www.bloomtechz.com/synthetic-chemical/peptide/cjc-1295-peptide-cas-863288-34-0.html
CJC-1295 Acetate : https://www.bloomtechz.com/synthetic-chemical/peptide/cjc-1295-acetate-cas-863288-34-0.html
1. Acidity and alkalinity:
CJC-1295 is a weakly acidic amino acid polypeptide with a pKa value of 4.8. This means that in an acidic environment below pH 4.8, CJC-1295 exists in an ionized form, while in a neutral or basic environment above pH 4.8, it exists in a non-ionized form.
It is also worth noting that if CJC-1295 is subjected to conditions such as heating or ultraviolet (UV) radiation, its molecules may be degraded, so care should be taken to select appropriate storage conditions and usage methods.
2. Oxidation-reduction properties:
The redox properties of CJC-1295 are mainly related to the sulfur bonds contained therein. Sulfur bonds can form stable disulfide bridges and can be reduced in the presence of reducing agents to change their molecular structures. For example, the cysteine amino acid in CJC-1295 was reduced to cysteine in the presence of sponge iron (FeS) catalyst.
In addition, CJC-1295 can also be oxidized, usually using hydrogen peroxide or other oxidizing agents as reagents. Since CJC-1295 is a polypeptide chain containing multiple amino acid residues, complex product mixtures may appear in the oxidation reaction, which need to be identified and analyzed by appropriate separation and purification methods.
3. Coordination reaction:
CJC-1295 contains many electrophilic atoms, such as nitrogen, oxygen and sulfur, which can form coordination bonds with metal ions. The coordination reaction between CJC-1295 and some metal ions has been extensively studied and it has been shown that this reaction has potential biological activity.
For example, CJC-1295 can coordinate with zinc ions to form a stable complex. This complex has the effect of promoting cell proliferation and repairing damaged tissue, so it may be expected to become a therapeutic agent.
4. Addition reaction:
CJC-1295 is also involved in various addition reactions in which the peptide bonds of CJC-1295 may be broken and form new functional groups. For example, CJC-1295 can generate free amino acids and condensation products of CJC-1295 through amide bond hydrolysis, which may have different biological activities.
In addition, CJC-1295 can also participate in allylation reaction, disulfide bond splitting reaction, etc. These reactions typically use reducing agents, peroxides, or other chemical reagents as reaction conditions. However, it should be noted that these reactions may lead to changes in the molecular structure of CJC-1295, thereby affecting its biological activity and efficacy.
5. Cyclization reaction:
CJC-1295 is a polypeptide chain containing 29 amino acids, many of which have reactive centers. Therefore, CJC-1295 may participate in various cyclization reactions to form new stable and efficient compounds. For example, CJC-1295 and C6 amino acids are linked by amide bonds to form a C6-CJC-1295 ring structure. This cyclized form is similar to the structure of a somatostatin-like peptide and may have a higher anti-growth hormone releasing hormone (GHRH) activity.
6. Enzyme hydrolysis reaction:
CJC-1295 may be attacked by enzymes such as protease and lyase in vivo, resulting in changes in molecular structure and reduction in biological activity. For example, under the action of trypsin, the peptide bonds in CJC-1295 may be hydrolyzed to produce catalytic products such as dipeptide or monopeptide, thereby affecting its efficacy.
In order to improve the bioavailability and efficacy of CJC-1295, researchers have developed a variety of stabilizers and protective agents, such as PEGylated and acetylated CJC-1295. These compounds have higher biological stability and longer survival time, and are expected to become more effective therapeutic drugs.
7. molecular structure:
CJC1295 is a polypeptide molecule composed of 29 amino acid residues, its sequence is Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln- Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2 (Y-D-(a-ADAIF)QSYRKVLAQLSARKLLDSR-NH2). Among them, CJC1295 uses D-amino acid, glycine dipeptide (Gly), disulfide bridge and other chemical modifications to increase its resistance to gastric acid and enzymes, thereby improving bioavailability and duration of drug effect.
8. Thermal stability:
CJC1295 can remain relatively stable within a certain temperature and time range. Studies have shown that when CJC1295 is dissolved in pure water or saline, the content loss rate is only about 1% after heating at 60°C for 30 minutes; and the content loss rate is about 10% after heating at 70°C for 30 minutes. Therefore, during production, storage and transportation, care should be taken to avoid exposure to extreme high or low temperature conditions, so as not to affect its efficacy.
9. pH stability:
The efficacy of CJC1295 is closely related to the pH value, and it is generally prone to failure in acidic environments. The experimental results show that the efficacy of CJC1295 has been destroyed after being dissolved in hydrochloric acid solution with a pH of 2.0 after 1 hour of treatment. In the buffer solution with pH 7.4, CJC1295 has higher biological activity and stability. Therefore, when using CJC1295, you should choose a buffer or additive that meets its drug efficacy needs, and control the pH value.
10. Solubility and stability:
CJC1295 is easily soluble in both water and ethanol, but its solubility in non-polar solvents is relatively low. In terms of its stability, CJC1295 is susceptible to factors such as oxidation, photolysis, and microbial contamination, so care should be taken to avoid prolonged exposure to air, sunlight, or microbial environments.
CJC-1295 has low water solubility and needs to be formulated by adding specific solvents. Common preparation solutions include anhydrous acetic acid, anhydrous methanol, PBS buffer, etc.
For the polypeptide chain of CJC-1295 containing DAC, it has more advantages in water solubility. After proper processing, CJC-1295 contains DAC which can be diluted with physiological saline (PBS) to form a clear solution.
11: Spectral features:
The infrared spectrum (IR) of CJC1295 shows that the absorption peaks are at 3296 cm^-1, 3084 cm^-1, 2926 cm^-1, 1623 cm^-1, 1538 cm^-1, 1395 cm^-1, 1329 The positions of cm^-1 and 1250 cm^-1 conform to the characteristics of peptide molecules. Its ultraviolet spectrum (UV) has an obvious absorption peak at 280 nm, indicating that it contains aromatic amino acid residues such as tyrosine and phenylalanine. The nuclear magnetic resonance (NMR) spectrum of CJC1295 showed complex signal patterns, reflecting the detailed information of its peptide structure.
As a GHRH analogue, CJC-1295 has broad clinical and non-clinical application prospects. Understanding the reactivity properties of CJC-1295 and understanding the interrelationship between its molecular structure and biological activity is crucial during drug development. With an in-depth understanding of the reactivity properties of CJC-1295, researchers can design more bioactive and stable derivatives and formulations, thereby improving its application prospects in the treatment and prevention of diseases.