Chlorophyllin powder, dark green water-soluble liquid or dark green powder. Easily soluble in water (slightly soluble in alcohol and chloroform, insoluble in grease. The aqueous solution is transparent, without precipitation). It uses natural green plant tissues, such as the leaves of silkworm excrement, clover, alfalfa, bamboo and other plants as raw materials, and extracts them with acetone, methanol, ethanol, petroleum ether and other organic solvents. Copper ions replace the magnesium ions in the chlorophyll center, and saponify them with alkali. The carboxyl formed after the removal of methyl and phytoalcohol groups becomes disodium salts. Therefore, sodium copper chlorophyllin is a semi synthetic pigment. The chlorophyll series pigments similar to its structure and formation principle include sodium iron chlorophyllin, sodium zinc chlorophyllin, etc.
|
Chemical Formula |
C34H31N4O65- |
|
Exact Mass |
203 |
|
Molecular Weight |
203 |
|
m/z |
118 (100.0%), 118 (36.8%), 119 (6.6%), 118 (1.5%), 119 (1.2%) |
|
Elemental Analysis |
C, 69.02; H, 5.28; N, 9.47; O, 16.22 |
|
|
|
1. The reaction mechanism is as follows:
The magnesium atom in the nucleus of chlorophyllin powder molecule combines with the nitrogen atom on the four pyrrole rings. One of the esters of dicarboxylic acid on the ring is esterified by alcohol and the other is esterified by leaf alcohol group, so saponification reaction can occur to generate sodium salt. (Take chlorophyll a as an example)
C55H72O5N4Mg+2NaOH - → C34H35O5N4MgNa2 + CH30H +C20H39OH
In acidic medium, magnesium in sodium chlorophyllin molecule is easily replaced by hydrogen atom to form brown demagnesium chlorophyllin acid.
C34H30O5N4MgNa2+4H+ → C34H34O5N4+ Mg2+ 2Na+
Demagnesic chlorophyll acid and copper salt can generate bright green chlorophyll keto acid under heating:
C34H34O5N4 + Cu2+- → C34H32O5N4Cu + 2H+
Dissolve chlorophyllin acid in acetone and react with alkali to form sodium copper chlorophyllin
C34H32O5N4Cu + 2NaOH- → C34H30O3N4Cu Na2 + H2O
2. Preparation process of sodium copper chlorophyllin:
Raw materials → leaching → filtering → concentration → saponification → separation → acid regulation → copper generation → filtering → salifying → drying → finished products
First, the raw materials are pretreated. At constant temperature, ethanol is used to extract the raw materials. After obtaining the dark green solution, NaOH solution is added for saponification, pH is adjusted, and constant temperature reflux is performed. After cooling, extract with petroleum ether. Adjust the pH of saponification solution, add CuSO under constant temperature, copper the solution, filter and wash for many times. Dissolve the precipitate with acetone, adjust the pH with NaOH solution until the crystals are separated out to obtain the product.
Characteristics: At present, this method is adopted in most literatures. However, due to the poor acid resistance and heat resistance of chlorophyll, the purity of the product obtained by this method is not high, and the entire experimental process needs to be improved.
According to the literature report, the extraction of chlorophyll mostly adopts conventional reflux method, Soxhlet extraction method and other means, but the extraction rate is low. Ultrasonic assisted technology is one of the most widely used in the field of natural plant extraction in recent years A new efficient extraction method. That is to say, ethanol is used as the extracting agent, ultrasonic wave is used to extract the raw materials for 20-30min, extracting for 2-3 times, and ultrasonic saponification for 20-30min. Other processes of this method are consistent with the route.
Characteristics: The ultrasonic method for preparing sodium copper chlorophyllin in accordance with the appropriate experimental conditions has the advantages of short time, low energy consumption, good stability and high extraction rate, which is obviously superior to the ordinary extraction and saponification methods.
This route is roughly the same as the route, but different in the extraction stage. Put the raw materials into the extraction kettle after pretreatment, seal, heat up and pump CO2 gas, add 90% ethanol as the entrainer, and extract chlorophyll. The fluid containing chlorophyll is separated by CO2 in a separating kettle to obtain the extract.
Features: Supercritical CO2 extraction can almost retain all effective ingredients in the material
High extraction efficiency, good selectivity, no damage to pigment components, no solvent residue, and high product purity
For convenience and other advantages.
After the raw materials are leached and saponified, copper ammonia complex is added into the saponified solution Stir and drop ammonia to adjust the pH, and then carry out copper reaction. After filtering the reaction solution, add it into the ethanol solution containing NaOH, adjust the pH to precipitate crystals, filter and dry to obtain dark green metallic luster copper sodium chlorophyll.
Features: This method has the advantages of high product yield, low ethanol consumption, low production cost, etc.
It provides an important basis for enterprises to produce sodium copper chlorophyllin.
Research on bioactive substances in plant-based foods has shown a close relationship between increasing consumption of fruits and vegetables and a decline in diseases such as cardiovascular disease and cancer. Chlorophyllin powder is one of the natural bioactive substances, and metalloporphyrins, as derivatives of chlorophyll, are the most unique among all natural pigments with a wide range of applications.
Due to the easy decomposition of natural chlorophyll under heat, light, acid, alkali, and its insolubility in water, its application is limited. Therefore, the structure of natural chlorophyll is modified to become a stable metalloporphyrin structure. The application fields of metalloporphyrins are constantly expanding and attracting much attention. Chlorophyll copper sodium salt, as a type of metalloporphyrin, has high stability and is widely used as a food additive, cosmetic additive, coloring agent, drug, photoelectric conversion material, and other fields. Chlorophyll copper sodium salt is derived from the conversion of chlorophyll, and natural chlorophyll has two structures, which makes its copper sodium salt more complex in composition and structure. In practice, expressing copper sodium salt only by molecular formula has its shortcomings compared to its wide application. Chlorophyll copper sodium salt has received special attention as a metalloporphyrin.
2. For textiles:
With the strengthening of people's awareness of environmental protection and the increasing emphasis on health, the negative effects of synthetic dyes used in textile dyeing on human health and the ecological environment have attracted more and more attention. Using pollution-free green natural dyes to dye textiles has become the research direction of many scholars. There are few natural dyes that can dye green, while sodium copper chlorophyllin is a food grade green color pigment and a natural chlorophyll derivative. It can be refined from the extracted chlorophyll through saponification, copper and other reactions. It is a highly stable metalloporphyrin, which is dark green powder with a slight metallic luster.
3. For cosmetics:
It can be added to cosmetics as a dye. Sodium copper chlorophyllin is a dark green powder, odorless or slightly odorous. The aqueous solution is transparent emerald green, which deepens with the increase of concentration. It is light resistant, heat resistant and stable. The pH of the 1% solution is 9.5~10.2. When the pH is below 6.5, it may precipitate when meeting calcium. Slightly soluble in ethanol. Acid drinks are easy to precipitate. Light resistance is stronger than chlorophyll, and it will decompose when heated to more than 110 ℃. In view of its stability and low toxicity, sodium copper chlorophyllin is widely used in the cosmetics industry.
4. Medical applications:
Applied research in the medical field has bright prospects because it has no toxic and side effects. The paste made of sodium copper chlorophyllin can accelerate wound healing when treating wounds. In daily life and clinical use as an air freshener, especially in anti-cancer and anti-tumor research is particularly prominent. It has been reported that various data on the effects of sodium copper chlorophyllin on human body are summarized in the form of detailed anti-tumor curves. The mechanisms of its direct or indirect anti-tumor effects mainly include the following aspects:
(1) Complexation with planar aromatic carcinogens;
(2) Inhibiting the activity of carcinogens;
(3) Degradation of carcinogens;
(4) Free radical scavenging and antioxidation. Some researchers have studied the components that play a role in sodium copper salt, and the results are the two with large content, but they have not pointed out which structure plays a role. Because sodium copper chlorophyllin has the function of scavenging free radicals, the study considered adding it to the cigarette filter tip to eliminate various free radicals in the smoke, thus reducing the harm to human body.
The development of organic dye sensitized wide band gap semiconductor photocell began in the 1990s. The photo electrode of the dye sensitized photoelectrochemical substrate model includes a porous large surface area semiconductor with a wide band gap, on which the dye sensitive to the visible spectrum is adsorbed. Due to the large surface area, porosity and other characteristics of ultra particle film (UPF), research on the construction of efficient photoelectrochemical substrates on UPF crystal electrodes has made rapid progress. Some researchers have prepared the photoelectrochemical substrate model of SnO2 ultrafine particle film, using sodium copper chlorophyllin as the sensitizer and I -/I3 - as the redox pair, designed and made the sandwich structure photoelectrochemical substrate, and studied its photoelectric conversion mechanism.
Specific steps
1. Adsorption of organic dyes by ultrafine particle films
+
-
Dissolve excess paste like sodium copper chlorophyllin powder in anhydrous ethanol and filter to obtain a bright green solution. Soak the conductive glass with SnO2 UPF deposited in this solution for 48 hours, then remove and air dry.
2. Production of photoelectrochemical substrates
+
-
The photoelectrochemical substrate is mainly composed of three parts: photoelectrode, electrolyte layer, and collector electrode, similar to a sandwich structure.
modular title
Photoelectrode:
It is prepared by depositing a layer of semiconductor UPF on conductive glass using direct current gas discharge activation reaction evaporation deposition method, and adsorbing dye molecules that are sensitive to light onto the UPF.
Electrolyte:
It contains redox pairs, which serve as a bridge between the photoelectrode and collector, and are a pathway for hole transfer in the battery circuit.
Collector:
The place in the electrolyte where electrons are regained through oxidation-reduction reactions that have lost electrons, must have good conductivity. Insulated enameled wire separates the photoelectrode and collector electrode, keeping them at a certain distance and providing some space for the electrolyte to exist.
Silver wires with strong conductivity are fixed on the photoelectrode and collector electrode respectively through conductive silver glue to connect external circuits and measure their open circuit voltage and short circuit current. The incident light is defined as normal incidence when it passes vertically through the collector, and as reverse incidence when it passes vertically through the photoelectrode.
The photosensitizer sodium copper chlorophyllin powder absorbs light, is activated, and then the activated sensitizer emits an electron to the conduction band of SnO2 semiconductor. The oxidized sensitizer is subsequently reduced by subsequent oxidation-reduction of the molecule. Subsequently, this successor molecule obtained electrons from the collector and regained neutrality. As a result, in an open circuit, the two electrodes generate a photoelectric potential, and if connected with an appropriate external circuit, there will be a response photoelectric current.
Frequently Asked Questions
What are the side effects of chlorophyllin?
+
-
Side effects of chlorophyllin supplementation may include green urine/stools, mild gastrointestinal upset, or photosensitivity. The risk for photosensitivity means that sun protection to prevent burns is important if you're using this supplement. Keep in mind that the supplement will also contain copper and sodium.
What does chlorophyll do to the body?
+
-
These extra minerals are there to make it easier for your body to absorb. The effects of chlorophyll are unclear. Supplement makers claim that chlorophyll can do many things, like boost red blood cells, help with weight loss, heal damaged skin, neutralize toxins, cut inflammation and prevent cancer.
What is the difference between chlorophyll and chlorophyllin?
+
-
Chlorophyll is ubiquitous in all green plant parts. Chlorophyllins are derivatives of chlorophyll in which the central magnesium atom is replaced by other metals, such as cobalt, copper or iron.
Hot Tags: chlorophyllin powder cas 11006-34-1, suppliers, manufacturers, factory, wholesale, buy, price, bulk, for sale, s allyl l cysteine, 5 hydroxymethylfurfural synthesis, Hinokitiol powder, spermidine powder, Pamoic acid